In vitro and in vivo tumoricidal properties of a pathogenic/free-living amoeba

Marsha S. Pidherney, Hassan Alizadeh, George L. Stewart, James P McCulley, Jerry Y Niederkorn

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The chemotactic and tumoricidal properties of the pathogenic/free-living amoeba Acanthamoeba castellanii were examined in vivo and in vitro. A. castellanii trophozoites displayed strong positive chemotactic responses to human melanoma (OCM-1) and murine melanoma (D5.1G4) cells. Although the parasites typically invade and destroy the corneal epithelium and stroma, positive chemotactic responses were not detected against extracts of either of these corneal elements. In vitro studies revealed that viable parasites, as well as cell-free parasite lysates, produced swift and extensive cytolysis of a wide variety of tumor cells. The tumoricidal properties of A. castellanii were examined in vivo and revealed that injection of viable parasites or cell-free parasite lysates into progressively growing subcutaneous melanomas resulted in 83% and 53% reductions in the tumor masses compared with untreated controls. The feasibility of utilizing the tumoricidal properties of pathogenic/free-living amoebae and their cell-free products in the treatment of drug-resistant or radioresistant tumors warrants further investigation.

Original languageEnglish (US)
Pages (from-to)91-98
Number of pages8
JournalCancer Letters
Volume72
Issue number1-2
DOIs
StatePublished - Aug 16 1993

Keywords

  • Acanthamoeba
  • cytolysis
  • free-living amoebae
  • melanoma

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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