TY - JOUR
T1 - In vitro correlates of Ld-restricted resistance to toxoplasmic encephalitis and their critical dependence on parasite strain
AU - Johnson, Jennifer J.
AU - Roberts, Craig W.
AU - Pope, Constance
AU - Roberts, Fiona
AU - Kirisits, Michael J.
AU - Estes, Randee
AU - Mui, Ernest
AU - Krieger, Tim
AU - Brown, Charles R.
AU - Forman, Jim
AU - McLeod, Rima
PY - 2002/7/15
Y1 - 2002/7/15
N2 - Resistance to murine toxoplasmic encephalitis has been precisely and definitively mapped to the Ld class I gene. Consistent with this, CD8+ T cells can adoptively transfer resistance to toxoplasmic encephalitis. However, cytotoxic CD8+ T cells, capable of killing class I-matched, infected target cells, are generated during the course of Toxoplasma gondii infection even in mice lacking the Ld gene. Ld-restricted killing could not be demonstrated, and the functional correlate of the Ld gene has therefore remained elusive. Herein, Ld-restricted killing of T. gondii-infected target cells is demonstrated for the first time. Ld-restricted killing is critically dependent on the strain of T. gondii and is observed with all the derivatives of type II strains tested, but not with a type I strain. These results have important implications for vaccine development.
AB - Resistance to murine toxoplasmic encephalitis has been precisely and definitively mapped to the Ld class I gene. Consistent with this, CD8+ T cells can adoptively transfer resistance to toxoplasmic encephalitis. However, cytotoxic CD8+ T cells, capable of killing class I-matched, infected target cells, are generated during the course of Toxoplasma gondii infection even in mice lacking the Ld gene. Ld-restricted killing could not be demonstrated, and the functional correlate of the Ld gene has therefore remained elusive. Herein, Ld-restricted killing of T. gondii-infected target cells is demonstrated for the first time. Ld-restricted killing is critically dependent on the strain of T. gondii and is observed with all the derivatives of type II strains tested, but not with a type I strain. These results have important implications for vaccine development.
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U2 - 10.4049/jimmunol.169.2.966
DO - 10.4049/jimmunol.169.2.966
M3 - Article
C2 - 12097403
AN - SCOPUS:0037098859
SN - 0022-1767
VL - 169
SP - 966
EP - 973
JO - Journal of Immunology
JF - Journal of Immunology
IS - 2
ER -