In vitro DNA mismatch repair in human cells

Liya Gu, Charles M. Ensor, Guo Min Li

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

The in vitro DNA mismatch repair (MMR) assay is a very useful technique for studying the functions and the mechanisms of the MMR system in genome maintenance. This assay has been effectively used to evaluate MMR proficiency in various tumor cells and to identify the majority of the protein components required for MMR. The procedure for setting up and performing the MMR assay involves mismatch substrate preparation, cell extract preparation, and the repair assay. In this chapter, we describe the detailed methods for this functional in vitro assay.

Original languageEnglish (US)
Title of host publicationDNA Repair Protocols
EditorsLotte Bjergbaek
Pages135-147
Number of pages13
DOIs
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
Volume920
ISSN (Print)1064-3745

Keywords

  • Column chromatography
  • DNA mismatch repair
  • Electrophoresis
  • HeLa-S3 nuclear extract
  • Mismatched DNA substrate

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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