Abstract
The in vitro DNA mismatch repair (MMR) assay is a very useful technique for studying the functions and the mechanisms of the MMR system in genome maintenance. This assay has been effectively used to evaluate MMR proficiency in various tumor cells and to identify the majority of the protein components required for MMR. The procedure for setting up and performing the MMR assay involves mismatch substrate preparation, cell extract preparation, and the repair assay. In this chapter, we describe the detailed methods for this functional in vitro assay.
| Original language | English (US) |
|---|---|
| Title of host publication | DNA Repair Protocols |
| Editors | Lotte Bjergbaek |
| Pages | 135-147 |
| Number of pages | 13 |
| DOIs | |
| State | Published - Nov 12 2012 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 920 |
| ISSN (Print) | 1064-3745 |
Keywords
- Column chromatography
- DNA mismatch repair
- Electrophoresis
- HeLa-S3 nuclear extract
- Mismatched DNA substrate
ASJC Scopus subject areas
- Molecular Biology
- Genetics
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Cite this
Gu, L., Ensor, C. M., & Li, G. M. (2012). In vitro DNA mismatch repair in human cells. In L. Bjergbaek (Ed.), DNA Repair Protocols (pp. 135-147). (Methods in Molecular Biology; Vol. 920). https://doi.org/10.1007/978-1-61779-998-3-10