In vitro DNA mismatch repair in human cells

Liya Gu; Charles M. Ensor; Guo Min Li

doi:10.1007/978-1-61779-998-3-10

In vitro DNA mismatch repair in human cells

Liya Gu, Charles M. Ensor, Guo Min Li

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

The in vitro DNA mismatch repair (MMR) assay is a very useful technique for studying the functions and the mechanisms of the MMR system in genome maintenance. This assay has been effectively used to evaluate MMR proficiency in various tumor cells and to identify the majority of the protein components required for MMR. The procedure for setting up and performing the MMR assay involves mismatch substrate preparation, cell extract preparation, and the repair assay. In this chapter, we describe the detailed methods for this functional in vitro assay.

Original language	English (US)
Title of host publication	DNA Repair Protocols
Pages	135-147
Number of pages	13
Volume	920
DOIs	https://doi.org/10.1007/978-1-61779-998-3-10
Publication status	Published - Nov 12 2012

Publication series

Name	Methods in Molecular Biology
Volume	920
ISSN (Print)	1064-3745

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Keywords

Column chromatography
DNA mismatch repair
Electrophoresis
HeLa-S3 nuclear extract
Mismatched DNA substrate

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Gu, L., Ensor, C. M., & Li, G. M. (2012). In vitro DNA mismatch repair in human cells. In DNA Repair Protocols (Vol. 920, pp. 135-147). (Methods in Molecular Biology; Vol. 920). https://doi.org/10.1007/978-1-61779-998-3-10

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Access to Document

10.1007/978-1-61779-998-3-10