In vitro growth inhibition of human small cell lung cancer by physalaemin

G. Bepler, D. N. Carney, A. F. Gazdar, J. D. Minna

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Production and secretion of neuroendocrine peptides by small cell lung cancer (SCLC) has been detected in the past years. Most recently the role of bombesin as an autocrine/paracrine growth modifier has been demonstrated. We used the soft agarose clonogenic assayd to evaluate the influence of other neuroendocrine peptides on the in vitro proliferation of SCLC cell lines. Neuroendocrine peptides tested were adrenocorticotrophic hormone, arginine vasopressin, calcitonin, glucagon, kassinin, neurotensin, physalaemin, somatostatin, and substance P. Experiments were carried out in serum-free and serum-supplemented media with and without serum-free incubation period. Our results indicated that the amphibian undecapeptide physalaemin inhibits the clonal and mass culture growth of SCLC cell lines at picomolar concentrations. All other neuroendocrine peptides failed to influence SCLC growth in the test systems used. These results suggest a growth regulating effect of physalaemin and a potential new form of neuroendocrine peptide therapy for SCLC.

Original languageEnglish (US)
Pages (from-to)2371-2375
Number of pages5
JournalCancer Research
Volume47
Issue number9
StatePublished - 1987

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Physalaemin
Small Cell Lung Carcinoma
Peptides
Growth
Kassinin
Cell Line
Bombesin
Neurotensin
Arginine Vasopressin
Serum-Free Culture Media
Calcitonin
Amphibians
Substance P
Somatostatin
Glucagon
Serum
Adrenocorticotropic Hormone
Sepharose
In Vitro Techniques

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

In vitro growth inhibition of human small cell lung cancer by physalaemin. / Bepler, G.; Carney, D. N.; Gazdar, A. F.; Minna, J. D.

In: Cancer Research, Vol. 47, No. 9, 1987, p. 2371-2375.

Research output: Contribution to journalArticle

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AU - Carney, D. N.

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AB - Production and secretion of neuroendocrine peptides by small cell lung cancer (SCLC) has been detected in the past years. Most recently the role of bombesin as an autocrine/paracrine growth modifier has been demonstrated. We used the soft agarose clonogenic assayd to evaluate the influence of other neuroendocrine peptides on the in vitro proliferation of SCLC cell lines. Neuroendocrine peptides tested were adrenocorticotrophic hormone, arginine vasopressin, calcitonin, glucagon, kassinin, neurotensin, physalaemin, somatostatin, and substance P. Experiments were carried out in serum-free and serum-supplemented media with and without serum-free incubation period. Our results indicated that the amphibian undecapeptide physalaemin inhibits the clonal and mass culture growth of SCLC cell lines at picomolar concentrations. All other neuroendocrine peptides failed to influence SCLC growth in the test systems used. These results suggest a growth regulating effect of physalaemin and a potential new form of neuroendocrine peptide therapy for SCLC.

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