In vitro intercellular adherence of Acanthamoeba castellanii: A scanning and transmission electron microscopy study

J. E. Ubelaker; M. B. Moore; J. H. Martin; R. Silveny; J. M. Dougherty; D. R. Meyer; J. P. McCulley

doi:10.1097/00003226-199107000-00004

In vitro intercellular adherence of Acanthamoeba castellanii: A scanning and transmission electron microscopy study

J. E. Ubelaker, M. B. Moore, J. H. Martin, R. Silveny, J. M. Dougherty, D. R. Meyer, J. P. McCulley

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Human corneal buttons were exposed to trophozoites and cysts of Acanthamoeba castellanii for 12 hours. Examination of the buttons by scanning electron microscopy showed numerous trophozoites on the surface of the epithelium. Trophozoites examined by transmission electron microscopy had limited regions of attachment to the epithelium but extensive regions of attachment to each other. Attachment regions were characterized as plaque-like maculae of an incomplete desmosome junction. Firm attachment mechanisms may explain how penetration of the human cornea occurs.

Original language	English (US)
Pages (from-to)	299-304
Number of pages	6
Journal	Cornea
Volume	10
Issue number	4
DOIs	https://doi.org/10.1097/00003226-199107000-00004
State	Published - 1991

ASJC Scopus subject areas

Ophthalmology

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10.1097/00003226-199107000-00004

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title = "In vitro intercellular adherence of Acanthamoeba castellanii: A scanning and transmission electron microscopy study",

abstract = "Human corneal buttons were exposed to trophozoites and cysts of Acanthamoeba castellanii for 12 hours. Examination of the buttons by scanning electron microscopy showed numerous trophozoites on the surface of the epithelium. Trophozoites examined by transmission electron microscopy had limited regions of attachment to the epithelium but extensive regions of attachment to each other. Attachment regions were characterized as plaque-like maculae of an incomplete desmosome junction. Firm attachment mechanisms may explain how penetration of the human cornea occurs.",

author = "Ubelaker, {J. E.} and Moore, {M. B.} and Martin, {J. H.} and R. Silveny and Dougherty, {J. M.} and Meyer, {D. R.} and McCulley, {J. P.}",

year = "1991",

doi = "10.1097/00003226-199107000-00004",

language = "English (US)",

volume = "10",

pages = "299--304",

journal = "Cornea",

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T1 - In vitro intercellular adherence of Acanthamoeba castellanii

T2 - A scanning and transmission electron microscopy study

AU - Ubelaker, J. E.

AU - Moore, M. B.

AU - Martin, J. H.

AU - Silveny, R.

AU - Dougherty, J. M.

AU - Meyer, D. R.

AU - McCulley, J. P.

PY - 1991

Y1 - 1991

N2 - Human corneal buttons were exposed to trophozoites and cysts of Acanthamoeba castellanii for 12 hours. Examination of the buttons by scanning electron microscopy showed numerous trophozoites on the surface of the epithelium. Trophozoites examined by transmission electron microscopy had limited regions of attachment to the epithelium but extensive regions of attachment to each other. Attachment regions were characterized as plaque-like maculae of an incomplete desmosome junction. Firm attachment mechanisms may explain how penetration of the human cornea occurs.

AB - Human corneal buttons were exposed to trophozoites and cysts of Acanthamoeba castellanii for 12 hours. Examination of the buttons by scanning electron microscopy showed numerous trophozoites on the surface of the epithelium. Trophozoites examined by transmission electron microscopy had limited regions of attachment to the epithelium but extensive regions of attachment to each other. Attachment regions were characterized as plaque-like maculae of an incomplete desmosome junction. Firm attachment mechanisms may explain how penetration of the human cornea occurs.

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EP - 304

JO - Cornea

JF - Cornea

IS - 4

ER -

In vitro intercellular adherence of Acanthamoeba castellanii: A scanning and transmission electron microscopy study

Abstract

ASJC Scopus subject areas

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