In vitro intercellular adherence of Acanthamoeba castellanii: A scanning and transmission electron microscopy study

J. E. Ubelaker, M. B. Moore, J. H. Martin, R. Silveny, J. M. Dougherty, D. R. Meyer, J. P. McCulley

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Human corneal buttons were exposed to trophozoites and cysts of Acanthamoeba castellanii for 12 hours. Examination of the buttons by scanning electron microscopy showed numerous trophozoites on the surface of the epithelium. Trophozoites examined by transmission electron microscopy had limited regions of attachment to the epithelium but extensive regions of attachment to each other. Attachment regions were characterized as plaque-like maculae of an incomplete desmosome junction. Firm attachment mechanisms may explain how penetration of the human cornea occurs.

Original languageEnglish (US)
Pages (from-to)299-304
Number of pages6
JournalCornea
Volume10
Issue number4
StatePublished - 1991

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Acanthamoeba castellanii
Trophozoites
Scanning Transmission Electron Microscopy
Epithelium
Desmosomes
Transmission Electron Microscopy
Electron Scanning Microscopy
Cornea
Cysts
In Vitro Techniques

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Ubelaker, J. E., Moore, M. B., Martin, J. H., Silveny, R., Dougherty, J. M., Meyer, D. R., & McCulley, J. P. (1991). In vitro intercellular adherence of Acanthamoeba castellanii: A scanning and transmission electron microscopy study. Cornea, 10(4), 299-304.

In vitro intercellular adherence of Acanthamoeba castellanii : A scanning and transmission electron microscopy study. / Ubelaker, J. E.; Moore, M. B.; Martin, J. H.; Silveny, R.; Dougherty, J. M.; Meyer, D. R.; McCulley, J. P.

In: Cornea, Vol. 10, No. 4, 1991, p. 299-304.

Research output: Contribution to journalArticle

Ubelaker, JE, Moore, MB, Martin, JH, Silveny, R, Dougherty, JM, Meyer, DR & McCulley, JP 1991, 'In vitro intercellular adherence of Acanthamoeba castellanii: A scanning and transmission electron microscopy study', Cornea, vol. 10, no. 4, pp. 299-304.
Ubelaker JE, Moore MB, Martin JH, Silveny R, Dougherty JM, Meyer DR et al. In vitro intercellular adherence of Acanthamoeba castellanii: A scanning and transmission electron microscopy study. Cornea. 1991;10(4):299-304.
Ubelaker, J. E. ; Moore, M. B. ; Martin, J. H. ; Silveny, R. ; Dougherty, J. M. ; Meyer, D. R. ; McCulley, J. P. / In vitro intercellular adherence of Acanthamoeba castellanii : A scanning and transmission electron microscopy study. In: Cornea. 1991 ; Vol. 10, No. 4. pp. 299-304.
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