In this report we describe methods which were used to cultivate cell lines from adenomatous polyps. Many of the techniques used have evolved from our past experience with the cultivation of colonic epithelial neoplastic cells (McBain et al., 1984) and the experience of others successful in the cultivation of benign and cancerous colon tissues. We feel that the preservation of cell-to-cell associations is a key to our successful cultivation of adenomas. Moyer (Moyer and Aust, 1984) has also pointed out the importance of preserving cell-to-cell associations in her successful studies to culture normal and neoplastic colonic tissues. Friedman's primary culture system for adenomatous polyps (Friedman et al., 1981) is further evidence supporting this point. Using the techniques described in this report, three cell lines, two small tubular polyps and one villous polyp, have been established and characterized. These cells can be expanded to numbers that will be sufficient for molecular and biochemical studies, and the cell number will be sufficient to conduct transfection experiments. Paraskeva (Paraskeva et al., 1984) has described two other cell lines derived from adenomatous polyps arising in patients with familial polyposis. The availability of cell lines from tubular and villous polyps make it possible to propose experiments to probe the biology of colonic adenomas.
|Original language||English (US)|
|Number of pages||10|
|Journal||Progress in Clinical and Biological Research|
|Publication status||Published - 1988|
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