In vitro production of angiotensin II by isolated glomeruli

B. A. Atiyeh, B. S. Arant, W. L. Henrich, M. G. Seikaly

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

The glomerulus has several components of the renin-angiotensin system (RAS). The purpose of this study was to evaluate the ability of glomeruli isolated from adult Wistar-Kyoto rats to produce angiotensin II (ANG II). When isolated glomeruli were incubated in Krebs buffer, the peak concentration of immunoreactive angiotensin (ANG) in the incubation medium, representing simultaneous production and degradation, occurred after 15 min of incubation (3.98 ± 0.34 pg·mg protein-1. 15 min-1), of which 18% was ANG II. When 125I-labeled ANG II was incubated with isolated glomeruli, the half-life of ANG II was 6.06 min. Hence, we estimated ANG II production at 3.77 ± 0.21 pg·mg protein-1. 15 min-1. When angiotensinogen-rich serum was added to the incubation medium, ANG concentration at 15 min increased by 500-fold (1,978 ± 44 pg·mg protein-1. 15 min-1, P < 0.001). ANG concentration in the glomerular incubate responded to perturbations known to alter systemic RAS. Enalaprilat, chymostatin, propranolol, and renin antiserum decreased ANG concentration in glomerular incubate, whereas salt depletion increased this (P < 0.05). We conclude that the rat glomerulus can generate ANG II independent of neural, hormonal, or vascular control.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Renal Fluid and Electrolyte Physiology
Volume268
Issue number2 37-2
StatePublished - Feb 1995

Fingerprint

angiotensin II
Angiotensin II
angiotensins
Angiotensins
renin-angiotensin system
Renin-Angiotensin System
angiotensinogen
Enalaprilat
Angiotensinogen
renin
propranolol
Proteins
Inbred WKY Rats
proteins
rats
blood serum
Renin
blood vessels
Propranolol
half life

Keywords

  • Angiotensinogen
  • Enalaprilat
  • Glomerulus
  • High-performance liquid chromatography
  • Propranolol
  • Radioimmunoassay
  • Rat
  • Renin
  • Renin antiserum

ASJC Scopus subject areas

  • Physiology
  • Agricultural and Biological Sciences(all)

Cite this

In vitro production of angiotensin II by isolated glomeruli. / Atiyeh, B. A.; Arant, B. S.; Henrich, W. L.; Seikaly, M. G.

In: American Journal of Physiology - Renal Fluid and Electrolyte Physiology, Vol. 268, No. 2 37-2, 02.1995.

Research output: Contribution to journalArticle

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