In Vitro Reconstitution of Autophagosome–Lysosome Fusion

J. Diao, L. Li, Y. Lai, Q. Zhong

Research output: Chapter in Book/Report/Conference proceedingChapter

4 Scopus citations

Abstract

SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) proteins are a highly regulated class of membrane proteins lying in the center of membrane fusion. In conjunction with accessory proteins, SNAREs drive efficient merger of two distinct lipid bilayers into one interconnected structure. This chapter describes our fluorescence resonance energy transfer (FRET)-based proteoliposome fusion assays for the roles of various SNARE proteins, accessory proteins, and effects of different lipid compositions on membrane fusion involved in autophagy.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages365-376
Number of pages12
Volume587
DOIs
Publication statusPublished - 2017

Publication series

NameMethods in Enzymology
Volume587
ISSN (Print)00766879
ISSN (Electronic)15577988

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Keywords

  • Accessory proteins
  • ATG14
  • Autophagy
  • Fusion
  • Lipid
  • Lysosome
  • Single vesicle
  • SNARE

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Diao, J., Li, L., Lai, Y., & Zhong, Q. (2017). In Vitro Reconstitution of Autophagosome–Lysosome Fusion. In Methods in Enzymology (Vol. 587, pp. 365-376). (Methods in Enzymology; Vol. 587). Academic Press Inc.. https://doi.org/10.1016/bs.mie.2016.09.065