In Vitro Signaling by MAPK and NFκB Pathways Inhibited by Yersinia YopJ

Sohini Mukherjee, Kim Orth

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A procedure for an in vitro signaling assay is described for the MAPK and NFκB pathways. The method uses a membrane-cleared lysate that contains all the soluble components required for activating these signaling cascades. The pathways can be activated by variety of molecules, including kinases, G-proteins, and E3 ligases. We demonstrate that YopJ inhibits downstream of all these activators. The in vitro signaling assay is ideal for initial biochemical studies on activators and inhibitors of the MAPK and NFκB pathways.

Original languageEnglish (US)
Pages (from-to)343-353
Number of pages11
JournalMethods in Enzymology
Volume438
DOIs
StatePublished - 2008

ASJC Scopus subject areas

  • Biochemistry

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