TY - JOUR
T1 - In Vitro Signaling by MAPK and NFκB Pathways Inhibited by Yersinia YopJ
AU - Mukherjee, Sohini
AU - Orth, Kim
PY - 2008
Y1 - 2008
N2 - A procedure for an in vitro signaling assay is described for the MAPK and NFκB pathways. The method uses a membrane-cleared lysate that contains all the soluble components required for activating these signaling cascades. The pathways can be activated by variety of molecules, including kinases, G-proteins, and E3 ligases. We demonstrate that YopJ inhibits downstream of all these activators. The in vitro signaling assay is ideal for initial biochemical studies on activators and inhibitors of the MAPK and NFκB pathways.
AB - A procedure for an in vitro signaling assay is described for the MAPK and NFκB pathways. The method uses a membrane-cleared lysate that contains all the soluble components required for activating these signaling cascades. The pathways can be activated by variety of molecules, including kinases, G-proteins, and E3 ligases. We demonstrate that YopJ inhibits downstream of all these activators. The in vitro signaling assay is ideal for initial biochemical studies on activators and inhibitors of the MAPK and NFκB pathways.
UR - http://www.scopus.com/inward/record.url?scp=45549093413&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=45549093413&partnerID=8YFLogxK
U2 - 10.1016/S0076-6879(07)38024-5
DO - 10.1016/S0076-6879(07)38024-5
M3 - Article
C2 - 18413260
AN - SCOPUS:45549093413
SN - 0076-6879
VL - 438
SP - 343
EP - 353
JO - Methods in Enzymology
JF - Methods in Enzymology
ER -