TY - JOUR
T1 - In vivo action of trefoil factor 2 (TFF2) to speed gastric repair is independent of cyclooxygenase
AU - Xue, Lin
AU - Aihara, Eitaro
AU - Podolsky, Daniel K.
AU - Wang, Timothy C.
AU - Montrose, Marshall H.
PY - 2010/9
Y1 - 2010/9
N2 - Objective: Trefoil factor (TFF) peptides are expressed in gastric tissues, where they are part of the epithelial defences. To complement previous in vitro work, the goal of the present study was to examine directly if TFF2 was essential for gastric restitution in vivo during the recovery from microscopic damage. Design: TFF2 mutant (K0) mice were examined to study the epithelial repair process in vivo after laser-induced photodamage (LPD). Using two-photon laser energy absorption (710 nm), LPD was imposed on an ~3-5 cell region of surface epithelium in anaesthetised mouse stomach. Responses to damage were evaluated during confocal time-lapse microscopy; including area of damage and the extracellular pH adjacent to the damaged surface (Cl-NERF pH sensor). Results In control (TFF2+/+ and TFF2+/-) mice, damaged cells were exfoliated and the damaged epithelium was repaired by indomethacin. The resting surface pH was similar between control and TFF2-K0 animals, but the post-LPD alkalisation of surface pH observed in control mice (ΔpH 0.3±0.05, n=21) was attenuated in the TFF2-K0 stomach (ΔpH -0.08±0.09, n=18). Recobinant rat TFF3 partially rescued the attenuated surface pH change in TFF2-K0 stomach, in the presence or absence of indomethacin. Conclusions: In the gastric epithelium in vivo, TFFs promote epithelial restitution via a mechanism that does not require cyclooxygenase activation. A novel role for TFFs to affect gastric surface pH is observed.
AB - Objective: Trefoil factor (TFF) peptides are expressed in gastric tissues, where they are part of the epithelial defences. To complement previous in vitro work, the goal of the present study was to examine directly if TFF2 was essential for gastric restitution in vivo during the recovery from microscopic damage. Design: TFF2 mutant (K0) mice were examined to study the epithelial repair process in vivo after laser-induced photodamage (LPD). Using two-photon laser energy absorption (710 nm), LPD was imposed on an ~3-5 cell region of surface epithelium in anaesthetised mouse stomach. Responses to damage were evaluated during confocal time-lapse microscopy; including area of damage and the extracellular pH adjacent to the damaged surface (Cl-NERF pH sensor). Results In control (TFF2+/+ and TFF2+/-) mice, damaged cells were exfoliated and the damaged epithelium was repaired by indomethacin. The resting surface pH was similar between control and TFF2-K0 animals, but the post-LPD alkalisation of surface pH observed in control mice (ΔpH 0.3±0.05, n=21) was attenuated in the TFF2-K0 stomach (ΔpH -0.08±0.09, n=18). Recobinant rat TFF3 partially rescued the attenuated surface pH change in TFF2-K0 stomach, in the presence or absence of indomethacin. Conclusions: In the gastric epithelium in vivo, TFFs promote epithelial restitution via a mechanism that does not require cyclooxygenase activation. A novel role for TFFs to affect gastric surface pH is observed.
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U2 - 10.1136/gut.2009.205625
DO - 10.1136/gut.2009.205625
M3 - Article
C2 - 20587547
AN - SCOPUS:77956100430
SN - 0017-5749
VL - 59
SP - 1184
EP - 1191
JO - Gut
JF - Gut
IS - 9
ER -