The effect of intravenous injection of Fab′ fragments of anti-L3T4 antibody (GK1.5 monoclonal antibody) into mice was studied. This treatment depleted L3T4+ cells from the popliteal lymph nodes of keyhole-limpet hemocyanin-primed mice. The T cells that remained were unable to provide help to antigen-specific B cells in vitro. The results obtained using Fab′ fragments were comparable with those using intact anti-L3T4 antibody and demonstrate that either form of GK1.5 is a potentially useful immunosuppressive agent in mice.
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