In vivo administration of Fab′ fragments of anti-L3T4 (GK1.5) antibody inhibits the T helper cell function of murine lymph node cells

Nancy E. Street, Koichi Uyemura, Virginia M. Sanders, Jonathan W. Uhr, Ellen S. Vitetta

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of intravenous injection of Fab′ fragments of anti-L3T4 antibody (GK1.5 monoclonal antibody) into mice was studied. This treatment depleted L3T4+ cells from the popliteal lymph nodes of keyhole-limpet hemocyanin-primed mice. The T cells that remained were unable to provide help to antigen-specific B cells in vitro. The results obtained using Fab′ fragments were comparable with those using intact anti-L3T4 antibody and demonstrate that either form of GK1.5 is a potentially useful immunosuppressive agent in mice.

Original languageEnglish (US)
Pages (from-to)75-81
Number of pages7
JournalCellular Immunology
Volume120
Issue number1
DOIs
StatePublished - Apr 15 1989

Fingerprint

Immunoglobulin Fab Fragments
Helper-Inducer T-Lymphocytes
Lymph Nodes
Anti-Idiotypic Antibodies
Immunosuppressive Agents
Intravenous Injections
B-Lymphocytes
T-Lymphocytes
Antigens
monoclonal antibody GK1.5
Therapeutics

ASJC Scopus subject areas

  • Cell Biology
  • Immunology

Cite this

In vivo administration of Fab′ fragments of anti-L3T4 (GK1.5) antibody inhibits the T helper cell function of murine lymph node cells. / Street, Nancy E.; Uyemura, Koichi; Sanders, Virginia M.; Uhr, Jonathan W.; Vitetta, Ellen S.

In: Cellular Immunology, Vol. 120, No. 1, 15.04.1989, p. 75-81.

Research output: Contribution to journalArticle

@article{736a3cc5b2b2465a981cdcfa83d67b77,
title = "In vivo administration of Fab′ fragments of anti-L3T4 (GK1.5) antibody inhibits the T helper cell function of murine lymph node cells",
abstract = "The effect of intravenous injection of Fab′ fragments of anti-L3T4 antibody (GK1.5 monoclonal antibody) into mice was studied. This treatment depleted L3T4+ cells from the popliteal lymph nodes of keyhole-limpet hemocyanin-primed mice. The T cells that remained were unable to provide help to antigen-specific B cells in vitro. The results obtained using Fab′ fragments were comparable with those using intact anti-L3T4 antibody and demonstrate that either form of GK1.5 is a potentially useful immunosuppressive agent in mice.",
author = "Street, {Nancy E.} and Koichi Uyemura and Sanders, {Virginia M.} and Uhr, {Jonathan W.} and Vitetta, {Ellen S.}",
year = "1989",
month = "4",
day = "15",
doi = "10.1016/0008-8749(89)90175-5",
language = "English (US)",
volume = "120",
pages = "75--81",
journal = "Cellular Immunology",
issn = "0008-8749",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - In vivo administration of Fab′ fragments of anti-L3T4 (GK1.5) antibody inhibits the T helper cell function of murine lymph node cells

AU - Street, Nancy E.

AU - Uyemura, Koichi

AU - Sanders, Virginia M.

AU - Uhr, Jonathan W.

AU - Vitetta, Ellen S.

PY - 1989/4/15

Y1 - 1989/4/15

N2 - The effect of intravenous injection of Fab′ fragments of anti-L3T4 antibody (GK1.5 monoclonal antibody) into mice was studied. This treatment depleted L3T4+ cells from the popliteal lymph nodes of keyhole-limpet hemocyanin-primed mice. The T cells that remained were unable to provide help to antigen-specific B cells in vitro. The results obtained using Fab′ fragments were comparable with those using intact anti-L3T4 antibody and demonstrate that either form of GK1.5 is a potentially useful immunosuppressive agent in mice.

AB - The effect of intravenous injection of Fab′ fragments of anti-L3T4 antibody (GK1.5 monoclonal antibody) into mice was studied. This treatment depleted L3T4+ cells from the popliteal lymph nodes of keyhole-limpet hemocyanin-primed mice. The T cells that remained were unable to provide help to antigen-specific B cells in vitro. The results obtained using Fab′ fragments were comparable with those using intact anti-L3T4 antibody and demonstrate that either form of GK1.5 is a potentially useful immunosuppressive agent in mice.

UR - http://www.scopus.com/inward/record.url?scp=0024563312&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024563312&partnerID=8YFLogxK

U2 - 10.1016/0008-8749(89)90175-5

DO - 10.1016/0008-8749(89)90175-5

M3 - Article

C2 - 2522831

AN - SCOPUS:0024563312

VL - 120

SP - 75

EP - 81

JO - Cellular Immunology

JF - Cellular Immunology

SN - 0008-8749

IS - 1

ER -