In vivo carbon nanotube-enhanced non-invasive photoacoustic mapping of the sentinel lymph node

Manojit Pramanik, Kwang Hyun Song, Magdalena Swierczewska, Danielle Green, Balaji Sitharaman, Lihong V. Wang

Research output: Contribution to journalArticle

98 Citations (Scopus)

Abstract

Sentinel lymph node biopsy (SLNB), a less invasive alternative to axillary lymph node dissection (ALND), has become the standard of care for patients with clinically node-negative breast cancer. In SLNB, lymphatic mapping with radio-labeled sulfur colloid and/or blue dye helps identify the sentinel lymph node (SLN), which is most likely to contain metastatic breast cancer. Even though SLNB, using both methylene blue and radioactive tracers, has a high identification rate, it still relies on an invasive surgical procedure, with associated morbidity. In this study, we have demonstrated a non-invasive single-walled carbon nanotube (SWNT)-enhanced photoacoustic (PA) identification of SLN in a rat model. We have successfully imaged the SLN in vivo by PA imaging (793 nm laser source, 5 MHz ultrasonic detector) with high contrast-to-noise ratio (=89) and good resolution (∼500 νm). The SWNTs also show a wideband optical absorption, generating PA signals over an excitation wavelength range of 740-820 nm. Thus, by varying the incident light wavelength to the near infrared region, where biological tissues (hemoglobin, tissue pigments, lipids and water) show low light absorption, the imaging depth is maximized. In the future, functionalization of the SWNTs with targeting groups should allow the molecular imaging of breast cancer.

Original languageEnglish (US)
Pages (from-to)3291-3301
Number of pages11
JournalPhysics in Medicine and Biology
Volume54
Issue number11
DOIs
StatePublished - 2009

Fingerprint

Sentinel Lymph Node Biopsy
Carbon Nanotubes
Breast Neoplasms
Radioactive Tracers
Molecular Imaging
Methylene Blue
Colloids
Standard of Care
Lymph Node Excision
Radio
Sulfur
Ultrasonics
Noise
Hemoglobins
Lasers
Coloring Agents
Morbidity
Lipids
Light
Water

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Pramanik, M., Song, K. H., Swierczewska, M., Green, D., Sitharaman, B., & Wang, L. V. (2009). In vivo carbon nanotube-enhanced non-invasive photoacoustic mapping of the sentinel lymph node. Physics in Medicine and Biology, 54(11), 3291-3301. https://doi.org/10.1088/0031-9155/54/11/001

In vivo carbon nanotube-enhanced non-invasive photoacoustic mapping of the sentinel lymph node. / Pramanik, Manojit; Song, Kwang Hyun; Swierczewska, Magdalena; Green, Danielle; Sitharaman, Balaji; Wang, Lihong V.

In: Physics in Medicine and Biology, Vol. 54, No. 11, 2009, p. 3291-3301.

Research output: Contribution to journalArticle

Pramanik, M, Song, KH, Swierczewska, M, Green, D, Sitharaman, B & Wang, LV 2009, 'In vivo carbon nanotube-enhanced non-invasive photoacoustic mapping of the sentinel lymph node', Physics in Medicine and Biology, vol. 54, no. 11, pp. 3291-3301. https://doi.org/10.1088/0031-9155/54/11/001
Pramanik, Manojit ; Song, Kwang Hyun ; Swierczewska, Magdalena ; Green, Danielle ; Sitharaman, Balaji ; Wang, Lihong V. / In vivo carbon nanotube-enhanced non-invasive photoacoustic mapping of the sentinel lymph node. In: Physics in Medicine and Biology. 2009 ; Vol. 54, No. 11. pp. 3291-3301.
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