In vivo confocal imaging: General principles and applications

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

It is well established that confocal microscopy provides higher resolution images with better rejection of out-of-focus information than conventional light microscopy. The optical sectioning ability of confocal microscopy allows images to be obtained from different depths within a thick tissue specimen, thereby eliminating the need for processing and sectioning procedures. Thus, confocal microscopy has made it possible to view biological tissues under more physiologic conditions than previously possible. The most widespread biological application of confocal microscopy has been in the localization of immunofluorescently labeled proteins in cell culture or within excised blocks of tissue. Because of its noninvasive optical sectioning capability, confocal microscopy is also ideally suited to the study of tissue in intact living animals, although the potential in vivo applications of this paradigm have received less attention. In this paper we trace the development of in vivo confocal microscopy and present examples of current capabilities for both research and clinical use.

Original languageEnglish (US)
Pages (from-to)131-149
Number of pages19
JournalScanning
Volume16
Issue number3
StatePublished - 1994

Fingerprint

Confocal microscopy
microscopy
Imaging techniques
Tissue
Image resolution
Cell culture
Optical microscopy
rejection
Animals
animals
Proteins
proteins
Processing
high resolution

Keywords

  • confocal microscopy
  • in vivo imaging
  • three-dimensional imaging
  • wound healing

ASJC Scopus subject areas

  • Instrumentation

Cite this

In vivo confocal imaging : General principles and applications. / Petroll, Walter M; Jester, J. V.; Cavanagh, Harrison D.

In: Scanning, Vol. 16, No. 3, 1994, p. 131-149.

Research output: Contribution to journalArticle

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