In Vivo Confocal Imaging of the eye Using Tandem Scanning Confocal Microscopy (TSCM)

James V. Jester, Harrison D Cavanagh, Michael A. Lemp

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

The tandem scanning reflected light microscope (TSRLM) is a confocal light microscope which has the capability of looking into living tissue and obtaining high resolution, high magnification images of cellular structure. TSRLM can be used to study living tissue such as all layers of the corneal epithelium including basal epithelial cells, keratocytes, nerves, inflammatory cells, bacteria, and corneal endothelium. For the first time in vision research, real-time, in vivo, microscopic images of normal and pathologic tissues can be obtained from human or animal eyes using the TSRLM. Compared to other methods of vital microscopy, TSRLM has no present rival. Specifically, TSRLM will: (1) Allow the hitopathologic analysis of living eyes, in vivo, over multiple observation periods without the need for tissue fixation and/or processing; (2) Assist in the acquisition and analysis of histopathologic images from human eyes, in vivo, in corneal disease; and (3) Greatly reduce the need for large numbers of animals in the histopathologic evaluation of experimental corneal disease and surgical procedures.

Original languageEnglish (US)
Pages (from-to)122-126
Number of pages5
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume1028
DOIs
StatePublished - Feb 9 1989

Fingerprint

Confocal Microscopy
Confocal
Confocal microscopy
Microscope
Scanning
Microscopes
microscopes
Imaging
microscopy
Imaging techniques
scanning
Tissue
animals
Animals
Endothelium
endothelium
epithelium
Cell
nerves
Fixation

ASJC Scopus subject areas

  • Applied Mathematics
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Electrical and Electronic Engineering
  • Computer Science Applications

Cite this

In Vivo Confocal Imaging of the eye Using Tandem Scanning Confocal Microscopy (TSCM). / Jester, James V.; Cavanagh, Harrison D; Lemp, Michael A.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 1028, 09.02.1989, p. 122-126.

Research output: Contribution to journalArticle

@article{50f49199aed1499f9aabe9339e373c8f,
title = "In Vivo Confocal Imaging of the eye Using Tandem Scanning Confocal Microscopy (TSCM)",
abstract = "The tandem scanning reflected light microscope (TSRLM) is a confocal light microscope which has the capability of looking into living tissue and obtaining high resolution, high magnification images of cellular structure. TSRLM can be used to study living tissue such as all layers of the corneal epithelium including basal epithelial cells, keratocytes, nerves, inflammatory cells, bacteria, and corneal endothelium. For the first time in vision research, real-time, in vivo, microscopic images of normal and pathologic tissues can be obtained from human or animal eyes using the TSRLM. Compared to other methods of vital microscopy, TSRLM has no present rival. Specifically, TSRLM will: (1) Allow the hitopathologic analysis of living eyes, in vivo, over multiple observation periods without the need for tissue fixation and/or processing; (2) Assist in the acquisition and analysis of histopathologic images from human eyes, in vivo, in corneal disease; and (3) Greatly reduce the need for large numbers of animals in the histopathologic evaluation of experimental corneal disease and surgical procedures.",
author = "Jester, {James V.} and Cavanagh, {Harrison D} and Lemp, {Michael A.}",
year = "1989",
month = "2",
day = "9",
doi = "10.1117/12.950323",
language = "English (US)",
volume = "1028",
pages = "122--126",
journal = "Proceedings of SPIE - The International Society for Optical Engineering",
issn = "0277-786X",
publisher = "SPIE",

}

TY - JOUR

T1 - In Vivo Confocal Imaging of the eye Using Tandem Scanning Confocal Microscopy (TSCM)

AU - Jester, James V.

AU - Cavanagh, Harrison D

AU - Lemp, Michael A.

PY - 1989/2/9

Y1 - 1989/2/9

N2 - The tandem scanning reflected light microscope (TSRLM) is a confocal light microscope which has the capability of looking into living tissue and obtaining high resolution, high magnification images of cellular structure. TSRLM can be used to study living tissue such as all layers of the corneal epithelium including basal epithelial cells, keratocytes, nerves, inflammatory cells, bacteria, and corneal endothelium. For the first time in vision research, real-time, in vivo, microscopic images of normal and pathologic tissues can be obtained from human or animal eyes using the TSRLM. Compared to other methods of vital microscopy, TSRLM has no present rival. Specifically, TSRLM will: (1) Allow the hitopathologic analysis of living eyes, in vivo, over multiple observation periods without the need for tissue fixation and/or processing; (2) Assist in the acquisition and analysis of histopathologic images from human eyes, in vivo, in corneal disease; and (3) Greatly reduce the need for large numbers of animals in the histopathologic evaluation of experimental corneal disease and surgical procedures.

AB - The tandem scanning reflected light microscope (TSRLM) is a confocal light microscope which has the capability of looking into living tissue and obtaining high resolution, high magnification images of cellular structure. TSRLM can be used to study living tissue such as all layers of the corneal epithelium including basal epithelial cells, keratocytes, nerves, inflammatory cells, bacteria, and corneal endothelium. For the first time in vision research, real-time, in vivo, microscopic images of normal and pathologic tissues can be obtained from human or animal eyes using the TSRLM. Compared to other methods of vital microscopy, TSRLM has no present rival. Specifically, TSRLM will: (1) Allow the hitopathologic analysis of living eyes, in vivo, over multiple observation periods without the need for tissue fixation and/or processing; (2) Assist in the acquisition and analysis of histopathologic images from human eyes, in vivo, in corneal disease; and (3) Greatly reduce the need for large numbers of animals in the histopathologic evaluation of experimental corneal disease and surgical procedures.

UR - http://www.scopus.com/inward/record.url?scp=84957522151&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957522151&partnerID=8YFLogxK

U2 - 10.1117/12.950323

DO - 10.1117/12.950323

M3 - Article

VL - 1028

SP - 122

EP - 126

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

SN - 0277-786X

ER -