In vivo dual-modality imaging of lymphatic systems using indocyanine green in rats: Three-dimensional photoacoustic imaging and planar fluorescence imaging

Chulhong Kim, Kwang Hyun Song, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

1 Scopus citations

Abstract

The purpose of this study is to map non-invasively sentinel lymph nodes (SLNs) and lymphatic vessels of rats in vivo using FDA-approved indocyanine green (ICG) and two non-ionizing imaging modalities: volumetric spectroscopic photoacoustic (PA) imaging, which measures optical absorption, and planar fluorescence imaging, which measures fluorescent emission. SLNs and lymphatic vessels were clearly visible after a 0.2 ml-intradermal-injection of 1 mM ICG in both imaging systems. We also imaged deeply positioned lymph nodes in vivo by layering biological tissues on top of rats. These two modalities, when used together with ICG, have the potential to map SLNs in axillary staging and to study tumor metastasis in breast cancer patients.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2010
DOIs
StatePublished - 2010
EventPhotons Plus Ultrasound: Imaging and Sensing 2010 - San Francisco, CA, United States
Duration: Jan 24 2010Jan 26 2010

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7564
ISSN (Print)1605-7422

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2010
Country/TerritoryUnited States
CitySan Francisco, CA
Period1/24/101/26/10

Keywords

  • Axillary lymph node dissection
  • Breast cancer
  • Photoacoustic tomography
  • Planar fluorescence imaging
  • Sentinel lymph node biopsy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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