In vivo enzymology: 13C NMR measurement of a kinetic isotope effect for methanol oxidation in Methylosinus trichosporium OB3b

Ralph P. Mason, Jeremy K M Sanders, Alex Cornish

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The competitive oxidation of 13CH3OH and 13CD3OH has been observed using in vivo 13C NMR spectroscopy. Simultaneous 1H and 2H decoupling gave isotopically shifted 13C singlets for the two methanol isotopomers. The measured enzymic isotope effect, kH/kD is approx. 1.8, indicating that CH bond cleavage is not rate-determining.

Original languageEnglish (US)
Pages (from-to)4-6
Number of pages3
JournalFEBS Letters
Volume216
Issue number1
DOIs
StatePublished - May 25 1987

Keywords

  • Enzyme kinetics
  • Formaldehyde production
  • Isotope effect
  • Methanol oxidation
  • NMR

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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