TY - JOUR
T1 - In vivo evaluation of the early events associated with liver metastasis of circulating cancer cells
AU - Ding, L.
AU - Sunamura, M.
AU - Kodama, T.
AU - Yamauchi, J.
AU - Duda, D. G.
AU - Shimamura, H.
AU - Shibuya, K.
AU - Takeda, K.
AU - Matsuno, S.
N1 - Funding Information:
We thank Emiko Shibuya and Hiroko Fujimura for their excellent technical assistance. This work was supported by a grant (05807094) from the Ministry of Education, Sports, Science, and Culture, Japan.
PY - 2001/8/3
Y1 - 2001/8/3
N2 - The mechanism of metastasis formation remains still largely unknown. Many studies underline the importance and complexity of the initial arrest of the circulating tumour cells in the target organ, a key stage in metastasis occurrence. In our study, we evaluated by visual means the metastasis formation using an in vivo microscopy system in a murine model. Moreover, we investigated the involvement of P-selectin in these processes using immunohistochemistry and P-selectin knockout mice. The present study offers direct evidence of distinct pathways for tumour metastasis formation by a lymphoma cell - EL-4 and a solid tumour cell - C26. Off-line analysis of the images and histological data confirmed that mechanical entrapment of the solid tumour cell, which had a bigger diameter than that of the liver sinusoids, promoted metastasis without any detectable involvement of adhesion molecules. On the other hand, we observed that lymphoma cells, in spite of their smaller diameter as compared to the sinusoids, promoted liver metastasis as well, but with the essential participation in their arrest of P-selectin, indicating an adhesion molecule-mediated pathway.
AB - The mechanism of metastasis formation remains still largely unknown. Many studies underline the importance and complexity of the initial arrest of the circulating tumour cells in the target organ, a key stage in metastasis occurrence. In our study, we evaluated by visual means the metastasis formation using an in vivo microscopy system in a murine model. Moreover, we investigated the involvement of P-selectin in these processes using immunohistochemistry and P-selectin knockout mice. The present study offers direct evidence of distinct pathways for tumour metastasis formation by a lymphoma cell - EL-4 and a solid tumour cell - C26. Off-line analysis of the images and histological data confirmed that mechanical entrapment of the solid tumour cell, which had a bigger diameter than that of the liver sinusoids, promoted metastasis without any detectable involvement of adhesion molecules. On the other hand, we observed that lymphoma cells, in spite of their smaller diameter as compared to the sinusoids, promoted liver metastasis as well, but with the essential participation in their arrest of P-selectin, indicating an adhesion molecule-mediated pathway.
KW - Circulating tumour cells
KW - Intravital microscopy
KW - Knockout mouse
KW - Liver microcirculation
KW - P-selectin
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U2 - 10.1054/bjoc.2001.1911
DO - 10.1054/bjoc.2001.1911
M3 - Article
C2 - 11487277
AN - SCOPUS:0035800444
SN - 0007-0920
VL - 85
SP - 431
EP - 438
JO - British journal of cancer
JF - British journal of cancer
IS - 3
ER -