In vivo evidence that ultraviolet B-induced suppression of allergic contact sensitivity is associated with functional inactivation of Th1 cells

J. C. Simon, T. Mosmann, D. Edelbaum, E. Schopf, P. R. Bergstresser, Ponciano D Cruz

Research output: Contribution to journalArticle

52 Citations (Scopus)

Abstract

Having previously shown in vitro that ultraviolet B (UVB)-treated Langerhans cells (LC) can induce antigen-specific proliferative unresponsiveness and tolerance in Th1 (but not Th2) cells, we wanted to determine whether cutaneous exposure to UVB radiation prior to hapten-painting would produce similar differential effects in hapten-reactive Th1 and Th2 T cells in vivo. C3H/HeN mice were exposed to UVB (200 J/m2/day) through abdominal skin on days -4 through -1, followed by painting dinitrofluorobenzene (DNFB) on the irradiated skin on days -1 and 0. Induction of allergic contact sensitivity (CS) was assayed by ear swelling responses to DNFB and by the proliferative responses of draining lymph node cells (LNC) to DNBS. UVB-irradiated and hapten-painted mice (in comparison to a control panel of unirradiated and DNFB-painted mice) displayed suppressed ear swelling responses to DNFB and suppressed LNC proliferation to DNBS, However, LNC from either panel of mice proliferated well in response to exogenous interleukin 2 (IL-2). To examine effects on Th1 and Th2 cells, lymphokines were assayed from supernatants of DNBS-stimulated LNC. The Th1-associated lymphokines, interferon-γ and IL-2, were the predominant cytokines detected in samples taken from unirradiated and DNFB-painted mice. Both of these cytokines were reduced markedly in samples from UVB-treated and DNFB-painted mice. Except for miniscule amounts of IL-10, no Th2-associated lymphokines were detected in LNC supernatants from either panel of mice. These results suggest that UVB-induced suppression of CS in vivo is associated with functional inactivation of hapten-reactive Th1 cells.

Original languageEnglish (US)
Pages (from-to)206-211
Number of pages6
JournalPhotodermatology Photoimmunology and Photomedicine
Volume10
Issue number5
StatePublished - 1994

Fingerprint

Dinitrofluorobenzene
Th1 Cells
Contact Dermatitis
Haptens
Lymph Nodes
Th2 Cells
Lymphokines
Paintings
Skin
Interleukin-2
Ear
Cytokines
Langerhans Cells
Inbred C3H Mouse
Interleukin-10
Interferons
Cell Proliferation
Radiation
T-Lymphocytes
Antigens

Keywords

  • Anergy
  • Immunosuppiession
  • Th1
  • Th2
  • Ultraviolet B

ASJC Scopus subject areas

  • Immunology
  • Dermatology

Cite this

In vivo evidence that ultraviolet B-induced suppression of allergic contact sensitivity is associated with functional inactivation of Th1 cells. / Simon, J. C.; Mosmann, T.; Edelbaum, D.; Schopf, E.; Bergstresser, P. R.; Cruz, Ponciano D.

In: Photodermatology Photoimmunology and Photomedicine, Vol. 10, No. 5, 1994, p. 206-211.

Research output: Contribution to journalArticle

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AU - Bergstresser, P. R.

AU - Cruz, Ponciano D

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