In vivo imaging of small animals with optical tomography and near infrared fluorescent probes

Matthew R. Palmer; Yasushi Shibata; Jonathan B. Kruskal; Robert E. Lenkinski

doi:10.1117/12.472078

In vivo imaging of small animals with optical tomography and near infrared fluorescent probes

Matthew R. Palmer, Yasushi Shibata, Jonathan B. Kruskal, Robert E. Lenkinski

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

5 Scopus citations

Abstract

A developmental optical tomograph has been designed for imaging small animals in vivo using near infrared (NIR) fluorphores. The system employs epi-illumnation via a 450 W Xe arc lamp, filtered and collimated to illuminate a 10 cm square movable stage. Emission light is filtered then collected by a high-resolution, high quantum efficiency, cooled CCD camera. Stage movement and image acquisition are under the control of a personal computer running system integration and automation software. During an experiment, the anesthetized animal is secured to the stage and up to 200 projections can be acquired over 180° rotation. Angular sampling of the light distribution at a point on the surface is used to determine relative contributions from ballistic and diffuse photons. We have employed the system to investigate a number of applications of in-vivo fluorescent imaging. In dynamic studies, hepatic function has been visualized in nude mice following intravenous injection of indocyanine green (ICG) and cerebrospinal fluid flow as been measured by injection of ICG-lipoprotein conjugate in the subarachnoid space of the lumbar spine followed by dynamic imaging of the brain. Further applications in physiological imaging, cancer detection, and molecular imaging are under investigation in our laboratory.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	D.J. Bornhop, D.A. Dunn, R.P. Mariella, Jr., C.J. Murphy, others and others
Pages	166-169
Number of pages	4
Volume	4626
DOIs	https://doi.org/10.1117/12.472078
State	Published - 2002
Event	Biomedical Nanotechnology Architectures and Applications - San Jose, CA, United States Duration: Jan 20 2002 → Jan 24 2002

Other

Other	Biomedical Nanotechnology Architectures and Applications
Country/Territory	United States
City	San Jose, CA
Period	1/20/02 → 1/24/02

Keywords

Fluorescence
Indocyanine green
Optical imaging

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

Access to Document

10.1117/12.472078

Cite this

Palmer, M. R., Shibata, Y., Kruskal, J. B., & Lenkinski, R. E. (2002). In vivo imaging of small animals with optical tomography and near infrared fluorescent probes. In D. J. Bornhop, D. A. Dunn, R. P. Mariella, Jr., C. J. Murphy, & O. and others (Eds.), Proceedings of SPIE - The International Society for Optical Engineering (Vol. 4626, pp. 166-169) https://doi.org/10.1117/12.472078

In vivo imaging of small animals with optical tomography and near infrared fluorescent probes. / Palmer, Matthew R.; Shibata, Yasushi; Kruskal, Jonathan B. et al.
Proceedings of SPIE - The International Society for Optical Engineering. ed. / D.J. Bornhop; D.A. Dunn; R.P. Mariella, Jr.; C.J. Murphy; others and others. Vol. 4626 2002. p. 166-169.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Palmer, MR, Shibata, Y, Kruskal, JB & Lenkinski, RE 2002, In vivo imaging of small animals with optical tomography and near infrared fluorescent probes. in DJ Bornhop, DA Dunn, RP Mariella, Jr., CJ Murphy & O and others (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 4626, pp. 166-169, Biomedical Nanotechnology Architectures and Applications, San Jose, CA, United States, 1/20/02. https://doi.org/10.1117/12.472078

@inproceedings{f74d3c361dc44ff5827725548a6b87e5,

title = "In vivo imaging of small animals with optical tomography and near infrared fluorescent probes",

abstract = "A developmental optical tomograph has been designed for imaging small animals in vivo using near infrared (NIR) fluorphores. The system employs epi-illumnation via a 450 W Xe arc lamp, filtered and collimated to illuminate a 10 cm square movable stage. Emission light is filtered then collected by a high-resolution, high quantum efficiency, cooled CCD camera. Stage movement and image acquisition are under the control of a personal computer running system integration and automation software. During an experiment, the anesthetized animal is secured to the stage and up to 200 projections can be acquired over 180° rotation. Angular sampling of the light distribution at a point on the surface is used to determine relative contributions from ballistic and diffuse photons. We have employed the system to investigate a number of applications of in-vivo fluorescent imaging. In dynamic studies, hepatic function has been visualized in nude mice following intravenous injection of indocyanine green (ICG) and cerebrospinal fluid flow as been measured by injection of ICG-lipoprotein conjugate in the subarachnoid space of the lumbar spine followed by dynamic imaging of the brain. Further applications in physiological imaging, cancer detection, and molecular imaging are under investigation in our laboratory.",

keywords = "Fluorescence, Indocyanine green, Optical imaging",

author = "Palmer, {Matthew R.} and Yasushi Shibata and Kruskal, {Jonathan B.} and Lenkinski, {Robert E.}",

year = "2002",

doi = "10.1117/12.472078",

language = "English (US)",

volume = "4626",

pages = "166--169",

editor = "D.J. Bornhop and D.A. Dunn and {Mariella, Jr.}, R.P. and C.J. Murphy and {and others}, others",

booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",

note = "Biomedical Nanotechnology Architectures and Applications ; Conference date: 20-01-2002 Through 24-01-2002",

}

TY - GEN

T1 - In vivo imaging of small animals with optical tomography and near infrared fluorescent probes

AU - Palmer, Matthew R.

AU - Shibata, Yasushi

AU - Kruskal, Jonathan B.

AU - Lenkinski, Robert E.

PY - 2002

Y1 - 2002

N2 - A developmental optical tomograph has been designed for imaging small animals in vivo using near infrared (NIR) fluorphores. The system employs epi-illumnation via a 450 W Xe arc lamp, filtered and collimated to illuminate a 10 cm square movable stage. Emission light is filtered then collected by a high-resolution, high quantum efficiency, cooled CCD camera. Stage movement and image acquisition are under the control of a personal computer running system integration and automation software. During an experiment, the anesthetized animal is secured to the stage and up to 200 projections can be acquired over 180° rotation. Angular sampling of the light distribution at a point on the surface is used to determine relative contributions from ballistic and diffuse photons. We have employed the system to investigate a number of applications of in-vivo fluorescent imaging. In dynamic studies, hepatic function has been visualized in nude mice following intravenous injection of indocyanine green (ICG) and cerebrospinal fluid flow as been measured by injection of ICG-lipoprotein conjugate in the subarachnoid space of the lumbar spine followed by dynamic imaging of the brain. Further applications in physiological imaging, cancer detection, and molecular imaging are under investigation in our laboratory.

AB - A developmental optical tomograph has been designed for imaging small animals in vivo using near infrared (NIR) fluorphores. The system employs epi-illumnation via a 450 W Xe arc lamp, filtered and collimated to illuminate a 10 cm square movable stage. Emission light is filtered then collected by a high-resolution, high quantum efficiency, cooled CCD camera. Stage movement and image acquisition are under the control of a personal computer running system integration and automation software. During an experiment, the anesthetized animal is secured to the stage and up to 200 projections can be acquired over 180° rotation. Angular sampling of the light distribution at a point on the surface is used to determine relative contributions from ballistic and diffuse photons. We have employed the system to investigate a number of applications of in-vivo fluorescent imaging. In dynamic studies, hepatic function has been visualized in nude mice following intravenous injection of indocyanine green (ICG) and cerebrospinal fluid flow as been measured by injection of ICG-lipoprotein conjugate in the subarachnoid space of the lumbar spine followed by dynamic imaging of the brain. Further applications in physiological imaging, cancer detection, and molecular imaging are under investigation in our laboratory.

KW - Fluorescence

KW - Indocyanine green

KW - Optical imaging

UR - http://www.scopus.com/inward/record.url?scp=0036407122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036407122&partnerID=8YFLogxK

U2 - 10.1117/12.472078

DO - 10.1117/12.472078

M3 - Conference contribution

AN - SCOPUS:0036407122

VL - 4626

SP - 166

EP - 169

BT - Proceedings of SPIE - The International Society for Optical Engineering

A2 - Bornhop, D.J.

A2 - Dunn, D.A.

A2 - Mariella, Jr., R.P.

A2 - Murphy, C.J.

A2 - and others, others

T2 - Biomedical Nanotechnology Architectures and Applications

Y2 - 20 January 2002 through 24 January 2002

ER -

In vivo imaging of small animals with optical tomography and near infrared fluorescent probes

Abstract

Other

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this