In Vivo Monitoring of Multi-Unit Neural Activity in the Suprachiasmatic Nucleus Reveals Robust Circadian Rhythms in Period1-/- Mice

Nana N. Takasu, Julie S. Pendergast, Cathya S. Olivas, Shin Yamazaki, Wataru Nakamura

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The master pacemaker in the suprachiasmatic nucleus (SCN) controls daily rhythms of behavior in mammals. C57BL/6J mice lacking Period1 (Per1-/-) are an anomaly because their SCN molecular rhythm is weak or absent in vitro even though their locomotor activity rhythm is robust. To resolve the contradiction between the in vitro and in vivo circadian phenotypes of Per1-/- mice, we measured the multi-unit activity (MUA) rhythm of the SCN neuronal population in freely-behaving mice. We found that in vivo Per1-/- SCN have high-amplitude MUA rhythms, demonstrating that the ensemble of neurons is driving robust locomotor activity in Per1-/- mice. Since the Per1-/- SCN electrical activity rhythm is indistinguishable from wild-types, in vivo physiological factors or coupling of the SCN to a known or unidentified circadian clock(s) may compensate for weak endogenous molecular rhythms in Per1-/- SCN. Consistent with the behavioral light responsiveness of Per1-/- mice, in vivo MUA rhythms in Per1-/- SCN exhibited large phase shifts in response to light. Since the acute response of the MUA rhythm to light in Per1-/- SCN is equivalent to wild-types, an unknown mechanism mediates enhanced light responsiveness of Per1-/- mice. Thus, Per1-/- mice are a unique model for investigating the component(s) of the in vivo environment that confers robust rhythmicity to the SCN as well as a novel mechanism of enhanced light responsiveness.

Original languageEnglish (US)
Article numbere64333
JournalPLoS One
Volume8
Issue number5
DOIs
StatePublished - May 22 2013

Fingerprint

Suprachiasmatic Nucleus
Circadian Rhythm
circadian rhythm
Monitoring
monitoring
mice
Light
locomotion
Pacemakers
Mammals
Locomotion
Phase shift
Neurons
Clocks
Circadian Clocks
neurons
Periodicity
mammals
Inbred C57BL Mouse
phenotype

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

In Vivo Monitoring of Multi-Unit Neural Activity in the Suprachiasmatic Nucleus Reveals Robust Circadian Rhythms in Period1-/- Mice. / Takasu, Nana N.; Pendergast, Julie S.; Olivas, Cathya S.; Yamazaki, Shin; Nakamura, Wataru.

In: PLoS One, Vol. 8, No. 5, e64333, 22.05.2013.

Research output: Contribution to journalArticle

Takasu, Nana N. ; Pendergast, Julie S. ; Olivas, Cathya S. ; Yamazaki, Shin ; Nakamura, Wataru. / In Vivo Monitoring of Multi-Unit Neural Activity in the Suprachiasmatic Nucleus Reveals Robust Circadian Rhythms in Period1-/- Mice. In: PLoS One. 2013 ; Vol. 8, No. 5.
@article{90ff3cc6890c496391ddbd35d4be53fa,
title = "In Vivo Monitoring of Multi-Unit Neural Activity in the Suprachiasmatic Nucleus Reveals Robust Circadian Rhythms in Period1-/- Mice",
abstract = "The master pacemaker in the suprachiasmatic nucleus (SCN) controls daily rhythms of behavior in mammals. C57BL/6J mice lacking Period1 (Per1-/-) are an anomaly because their SCN molecular rhythm is weak or absent in vitro even though their locomotor activity rhythm is robust. To resolve the contradiction between the in vitro and in vivo circadian phenotypes of Per1-/- mice, we measured the multi-unit activity (MUA) rhythm of the SCN neuronal population in freely-behaving mice. We found that in vivo Per1-/- SCN have high-amplitude MUA rhythms, demonstrating that the ensemble of neurons is driving robust locomotor activity in Per1-/- mice. Since the Per1-/- SCN electrical activity rhythm is indistinguishable from wild-types, in vivo physiological factors or coupling of the SCN to a known or unidentified circadian clock(s) may compensate for weak endogenous molecular rhythms in Per1-/- SCN. Consistent with the behavioral light responsiveness of Per1-/- mice, in vivo MUA rhythms in Per1-/- SCN exhibited large phase shifts in response to light. Since the acute response of the MUA rhythm to light in Per1-/- SCN is equivalent to wild-types, an unknown mechanism mediates enhanced light responsiveness of Per1-/- mice. Thus, Per1-/- mice are a unique model for investigating the component(s) of the in vivo environment that confers robust rhythmicity to the SCN as well as a novel mechanism of enhanced light responsiveness.",
author = "Takasu, {Nana N.} and Pendergast, {Julie S.} and Olivas, {Cathya S.} and Shin Yamazaki and Wataru Nakamura",
year = "2013",
month = "5",
day = "22",
doi = "10.1371/journal.pone.0064333",
language = "English (US)",
volume = "8",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "5",

}

TY - JOUR

T1 - In Vivo Monitoring of Multi-Unit Neural Activity in the Suprachiasmatic Nucleus Reveals Robust Circadian Rhythms in Period1-/- Mice

AU - Takasu, Nana N.

AU - Pendergast, Julie S.

AU - Olivas, Cathya S.

AU - Yamazaki, Shin

AU - Nakamura, Wataru

PY - 2013/5/22

Y1 - 2013/5/22

N2 - The master pacemaker in the suprachiasmatic nucleus (SCN) controls daily rhythms of behavior in mammals. C57BL/6J mice lacking Period1 (Per1-/-) are an anomaly because their SCN molecular rhythm is weak or absent in vitro even though their locomotor activity rhythm is robust. To resolve the contradiction between the in vitro and in vivo circadian phenotypes of Per1-/- mice, we measured the multi-unit activity (MUA) rhythm of the SCN neuronal population in freely-behaving mice. We found that in vivo Per1-/- SCN have high-amplitude MUA rhythms, demonstrating that the ensemble of neurons is driving robust locomotor activity in Per1-/- mice. Since the Per1-/- SCN electrical activity rhythm is indistinguishable from wild-types, in vivo physiological factors or coupling of the SCN to a known or unidentified circadian clock(s) may compensate for weak endogenous molecular rhythms in Per1-/- SCN. Consistent with the behavioral light responsiveness of Per1-/- mice, in vivo MUA rhythms in Per1-/- SCN exhibited large phase shifts in response to light. Since the acute response of the MUA rhythm to light in Per1-/- SCN is equivalent to wild-types, an unknown mechanism mediates enhanced light responsiveness of Per1-/- mice. Thus, Per1-/- mice are a unique model for investigating the component(s) of the in vivo environment that confers robust rhythmicity to the SCN as well as a novel mechanism of enhanced light responsiveness.

AB - The master pacemaker in the suprachiasmatic nucleus (SCN) controls daily rhythms of behavior in mammals. C57BL/6J mice lacking Period1 (Per1-/-) are an anomaly because their SCN molecular rhythm is weak or absent in vitro even though their locomotor activity rhythm is robust. To resolve the contradiction between the in vitro and in vivo circadian phenotypes of Per1-/- mice, we measured the multi-unit activity (MUA) rhythm of the SCN neuronal population in freely-behaving mice. We found that in vivo Per1-/- SCN have high-amplitude MUA rhythms, demonstrating that the ensemble of neurons is driving robust locomotor activity in Per1-/- mice. Since the Per1-/- SCN electrical activity rhythm is indistinguishable from wild-types, in vivo physiological factors or coupling of the SCN to a known or unidentified circadian clock(s) may compensate for weak endogenous molecular rhythms in Per1-/- SCN. Consistent with the behavioral light responsiveness of Per1-/- mice, in vivo MUA rhythms in Per1-/- SCN exhibited large phase shifts in response to light. Since the acute response of the MUA rhythm to light in Per1-/- SCN is equivalent to wild-types, an unknown mechanism mediates enhanced light responsiveness of Per1-/- mice. Thus, Per1-/- mice are a unique model for investigating the component(s) of the in vivo environment that confers robust rhythmicity to the SCN as well as a novel mechanism of enhanced light responsiveness.

UR - http://www.scopus.com/inward/record.url?scp=84878039907&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878039907&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0064333

DO - 10.1371/journal.pone.0064333

M3 - Article

C2 - 23717599

AN - SCOPUS:84878039907

VL - 8

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 5

M1 - e64333

ER -