In vivo near-infrared fluorescence imaging of osteoblastic activity

Atif Zaheer, Robert E. Lenkinski, Ashfaq Mahmood, Alun G. Jones, Lewis C. Cantley, John V. Frangioni

Research output: Contribution to journalArticle

291 Citations (Scopus)

Abstract

In vertebrates, the development and integrity of the skeleton requires hydroxyapatite (HA) deposition by osteoblasts. HA deposition is also a marker of, or a participant in, processes as diverse as cancer and atherosclerosis. At present, sites of osteoblastic activity can only be imaged in vivo using γ-emitting radioisotopes. The scan times required are long, and the resultant radioscintigraphic images suffer from relatively low resolution. We have synthesized a near-infrared (NIR) fluorescent bisphosphonate derivative that exhibits rapid and specific binding to HA in vitro and in vivo. We demonstrate NIR light-based detection of osteoblastic activity in the living animal, and discuss how this technology can be used to study skeletal development, osteoblastic metastasis, coronary atherosclerosis, and other human diseases.

Original languageEnglish (US)
Pages (from-to)1148-1154
Number of pages7
JournalNature Biotechnology
Volume19
Issue number12
DOIs
StatePublished - 2001

Fingerprint

Optical Imaging
Durapatite
Hydroxyapatite
Fluorescence
Infrared radiation
Imaging techniques
Osteoblasts
Diphosphonates
Radioisotopes
Skeleton
Vertebrates
Coronary Artery Disease
Atherosclerosis
Animals
Neoplasm Metastasis
Technology
Derivatives
Light
Neoplasms

ASJC Scopus subject areas

  • Microbiology

Cite this

Zaheer, A., Lenkinski, R. E., Mahmood, A., Jones, A. G., Cantley, L. C., & Frangioni, J. V. (2001). In vivo near-infrared fluorescence imaging of osteoblastic activity. Nature Biotechnology, 19(12), 1148-1154. https://doi.org/10.1038/nbt1201-1148

In vivo near-infrared fluorescence imaging of osteoblastic activity. / Zaheer, Atif; Lenkinski, Robert E.; Mahmood, Ashfaq; Jones, Alun G.; Cantley, Lewis C.; Frangioni, John V.

In: Nature Biotechnology, Vol. 19, No. 12, 2001, p. 1148-1154.

Research output: Contribution to journalArticle

Zaheer, A, Lenkinski, RE, Mahmood, A, Jones, AG, Cantley, LC & Frangioni, JV 2001, 'In vivo near-infrared fluorescence imaging of osteoblastic activity', Nature Biotechnology, vol. 19, no. 12, pp. 1148-1154. https://doi.org/10.1038/nbt1201-1148
Zaheer A, Lenkinski RE, Mahmood A, Jones AG, Cantley LC, Frangioni JV. In vivo near-infrared fluorescence imaging of osteoblastic activity. Nature Biotechnology. 2001;19(12):1148-1154. https://doi.org/10.1038/nbt1201-1148
Zaheer, Atif ; Lenkinski, Robert E. ; Mahmood, Ashfaq ; Jones, Alun G. ; Cantley, Lewis C. ; Frangioni, John V. / In vivo near-infrared fluorescence imaging of osteoblastic activity. In: Nature Biotechnology. 2001 ; Vol. 19, No. 12. pp. 1148-1154.
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