In vivo near-infrared fluorescence imaging of osteoblastic activity

Atif Zaheer; Robert E. Lenkinski; Ashfaq Mahmood; Alun G. Jones; Lewis C. Cantley; John V. Frangioni

doi:10.1038/nbt1201-1148

In vivo near-infrared fluorescence imaging of osteoblastic activity

Atif Zaheer, Robert E. Lenkinski, Ashfaq Mahmood, Alun G. Jones, Lewis C. Cantley, John V. Frangioni

Research output: Contribution to journal › Article › peer-review

301 Scopus citations

Abstract

In vertebrates, the development and integrity of the skeleton requires hydroxyapatite (HA) deposition by osteoblasts. HA deposition is also a marker of, or a participant in, processes as diverse as cancer and atherosclerosis. At present, sites of osteoblastic activity can only be imaged in vivo using γ-emitting radioisotopes. The scan times required are long, and the resultant radioscintigraphic images suffer from relatively low resolution. We have synthesized a near-infrared (NIR) fluorescent bisphosphonate derivative that exhibits rapid and specific binding to HA in vitro and in vivo. We demonstrate NIR light-based detection of osteoblastic activity in the living animal, and discuss how this technology can be used to study skeletal development, osteoblastic metastasis, coronary atherosclerosis, and other human diseases.

Original language	English (US)
Pages (from-to)	1148-1154
Number of pages	7
Journal	Nature biotechnology
Volume	19
Issue number	12
DOIs	https://doi.org/10.1038/nbt1201-1148
State	Published - Dec 17 2001

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology
Molecular Medicine
Biomedical Engineering

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title = "In vivo near-infrared fluorescence imaging of osteoblastic activity",

abstract = "In vertebrates, the development and integrity of the skeleton requires hydroxyapatite (HA) deposition by osteoblasts. HA deposition is also a marker of, or a participant in, processes as diverse as cancer and atherosclerosis. At present, sites of osteoblastic activity can only be imaged in vivo using γ-emitting radioisotopes. The scan times required are long, and the resultant radioscintigraphic images suffer from relatively low resolution. We have synthesized a near-infrared (NIR) fluorescent bisphosphonate derivative that exhibits rapid and specific binding to HA in vitro and in vivo. We demonstrate NIR light-based detection of osteoblastic activity in the living animal, and discuss how this technology can be used to study skeletal development, osteoblastic metastasis, coronary atherosclerosis, and other human diseases.",

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AU - Zaheer, Atif

AU - Lenkinski, Robert E.

AU - Mahmood, Ashfaq

AU - Jones, Alun G.

AU - Cantley, Lewis C.

AU - Frangioni, John V.

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