Abstract
In vertebrates, the development and integrity of the skeleton requires hydroxyapatite (HA) deposition by osteoblasts. HA deposition is also a marker of, or a participant in, processes as diverse as cancer and atherosclerosis. At present, sites of osteoblastic activity can only be imaged in vivo using γ-emitting radioisotopes. The scan times required are long, and the resultant radioscintigraphic images suffer from relatively low resolution. We have synthesized a near-infrared (NIR) fluorescent bisphosphonate derivative that exhibits rapid and specific binding to HA in vitro and in vivo. We demonstrate NIR light-based detection of osteoblastic activity in the living animal, and discuss how this technology can be used to study skeletal development, osteoblastic metastasis, coronary atherosclerosis, and other human diseases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1148-1154 |
| Number of pages | 7 |
| Journal | Nature Biotechnology |
| Volume | 19 |
| Issue number | 12 |
| DOIs | |
| State | Published - 2001 |
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ASJC Scopus subject areas
- Microbiology
Cite this
In vivo near-infrared fluorescence imaging of osteoblastic activity. / Zaheer, Atif; Lenkinski, Robert E.; Mahmood, Ashfaq; Jones, Alun G.; Cantley, Lewis C.; Frangioni, John V.
In: Nature Biotechnology, Vol. 19, No. 12, 2001, p. 1148-1154.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - In vivo near-infrared fluorescence imaging of osteoblastic activity
AU - Zaheer, Atif
AU - Lenkinski, Robert E.
AU - Mahmood, Ashfaq
AU - Jones, Alun G.
AU - Cantley, Lewis C.
AU - Frangioni, John V.
PY - 2001
Y1 - 2001
N2 - In vertebrates, the development and integrity of the skeleton requires hydroxyapatite (HA) deposition by osteoblasts. HA deposition is also a marker of, or a participant in, processes as diverse as cancer and atherosclerosis. At present, sites of osteoblastic activity can only be imaged in vivo using γ-emitting radioisotopes. The scan times required are long, and the resultant radioscintigraphic images suffer from relatively low resolution. We have synthesized a near-infrared (NIR) fluorescent bisphosphonate derivative that exhibits rapid and specific binding to HA in vitro and in vivo. We demonstrate NIR light-based detection of osteoblastic activity in the living animal, and discuss how this technology can be used to study skeletal development, osteoblastic metastasis, coronary atherosclerosis, and other human diseases.
AB - In vertebrates, the development and integrity of the skeleton requires hydroxyapatite (HA) deposition by osteoblasts. HA deposition is also a marker of, or a participant in, processes as diverse as cancer and atherosclerosis. At present, sites of osteoblastic activity can only be imaged in vivo using γ-emitting radioisotopes. The scan times required are long, and the resultant radioscintigraphic images suffer from relatively low resolution. We have synthesized a near-infrared (NIR) fluorescent bisphosphonate derivative that exhibits rapid and specific binding to HA in vitro and in vivo. We demonstrate NIR light-based detection of osteoblastic activity in the living animal, and discuss how this technology can be used to study skeletal development, osteoblastic metastasis, coronary atherosclerosis, and other human diseases.
UR - http://www.scopus.com/inward/record.url?scp=0035200283&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0035200283&partnerID=8YFLogxK
U2 - 10.1038/nbt1201-1148
DO - 10.1038/nbt1201-1148
M3 - Article
C2 - 11731784
AN - SCOPUS:0035200283
VL - 19
SP - 1148
EP - 1154
JO - Biotechnology
JF - Biotechnology
SN - 0733-222X
IS - 12
ER -