In vivo photoacoustic (PA) mapping of sentinel lymph nodes (SLNs) using carbon nanotubes (CNTs) as a contrast agent

Manojit Pramanik, Kwang Hyun Song, Magdalena Swierczewska, Danielle Green, Balaji Sitharaman, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Sentinel lymph node biopsy (SLNB), a less invasive alternative to axillary lymph node dissection (ALND), is routinely used in clinic for staging breast cancer. In SLNB, lymphatic mapping with radio-labeled sulfur colloid and/or blue dye helps identify the sentinel lymph node (SLN), which is most likely to contain metastatic breast cancer. Even though SLNB, using both methylene blue and radioactive tracers, has a high identification rate, it still relies on an invasive surgical procedure, with associated morbidity. In this study, we have demonstrated a non-invasive single-walled carbon nanotube (SWNT)-enhanced photoacoustic (PA) identification of SLN in a rat model. We have used single-walled carbon nanotubes (SWNTs) as a photoacoustic contrast agent to map non-invasively the sentinel lymph nodes (SLNs) in a rat model in vivo. We were able to identify the SLN non-invasively with high contrast to noise ratio (∼90) and high resolution (∼500 μm). Due to the broad photoacoustic spectrum of these nanotubes in the near infrared wavelength window we could easily choose a suitable light wavelength to maximize the imaging depth. Our results suggest that this technology could be a useful clinical tool, allowing clinicians to identify SLNs non-invasively in vivo. In the future, these contrast agents could be functionalized to do molecular photoacoustic imaging.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7177
DOIs
StatePublished - 2009
EventPhotons Plus Ultrasound: Imaging and Sensing 2009 - San Jose, CA, United States
Duration: Jan 25 2009Jan 28 2009

Other

OtherPhotons Plus Ultrasound: Imaging and Sensing 2009
CountryUnited States
CitySan Jose, CA
Period1/25/091/28/09

Fingerprint

Carbon Nanotubes
Photoacoustic effect
lymphatic system
Nanotubes
Contrast Media
Biopsy
Carbon nanotubes
Carbon
carbon nanotubes
Single-walled carbon nanotubes (SWCN)
Vertex of a graph
Rats
Imaging techniques
Dissection
Radioactive tracers
Wavelength
Single-walled Carbon Nanotubes
Colloids
Breast Cancer
breast

Keywords

  • Axillary lymph node dissection
  • Breast cancer
  • Carbon nanotube
  • Photoacoustic tomography
  • Sentinel lymph node biopsy
  • Single-walled carbon nanotube

ASJC Scopus subject areas

  • Applied Mathematics
  • Computer Science Applications
  • Electrical and Electronic Engineering
  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics

Cite this

Pramanik, M., Song, K. H., Swierczewska, M., Green, D., Sitharaman, B., & Wang, L. V. (2009). In vivo photoacoustic (PA) mapping of sentinel lymph nodes (SLNs) using carbon nanotubes (CNTs) as a contrast agent. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 7177). [71771N] https://doi.org/10.1117/12.808522

In vivo photoacoustic (PA) mapping of sentinel lymph nodes (SLNs) using carbon nanotubes (CNTs) as a contrast agent. / Pramanik, Manojit; Song, Kwang Hyun; Swierczewska, Magdalena; Green, Danielle; Sitharaman, Balaji; Wang, Lihong V.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7177 2009. 71771N.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pramanik, M, Song, KH, Swierczewska, M, Green, D, Sitharaman, B & Wang, LV 2009, In vivo photoacoustic (PA) mapping of sentinel lymph nodes (SLNs) using carbon nanotubes (CNTs) as a contrast agent. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 7177, 71771N, Photons Plus Ultrasound: Imaging and Sensing 2009, San Jose, CA, United States, 1/25/09. https://doi.org/10.1117/12.808522
Pramanik M, Song KH, Swierczewska M, Green D, Sitharaman B, Wang LV. In vivo photoacoustic (PA) mapping of sentinel lymph nodes (SLNs) using carbon nanotubes (CNTs) as a contrast agent. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7177. 2009. 71771N https://doi.org/10.1117/12.808522
Pramanik, Manojit ; Song, Kwang Hyun ; Swierczewska, Magdalena ; Green, Danielle ; Sitharaman, Balaji ; Wang, Lihong V. / In vivo photoacoustic (PA) mapping of sentinel lymph nodes (SLNs) using carbon nanotubes (CNTs) as a contrast agent. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 7177 2009.
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abstract = "Sentinel lymph node biopsy (SLNB), a less invasive alternative to axillary lymph node dissection (ALND), is routinely used in clinic for staging breast cancer. In SLNB, lymphatic mapping with radio-labeled sulfur colloid and/or blue dye helps identify the sentinel lymph node (SLN), which is most likely to contain metastatic breast cancer. Even though SLNB, using both methylene blue and radioactive tracers, has a high identification rate, it still relies on an invasive surgical procedure, with associated morbidity. In this study, we have demonstrated a non-invasive single-walled carbon nanotube (SWNT)-enhanced photoacoustic (PA) identification of SLN in a rat model. We have used single-walled carbon nanotubes (SWNTs) as a photoacoustic contrast agent to map non-invasively the sentinel lymph nodes (SLNs) in a rat model in vivo. We were able to identify the SLN non-invasively with high contrast to noise ratio (∼90) and high resolution (∼500 μm). Due to the broad photoacoustic spectrum of these nanotubes in the near infrared wavelength window we could easily choose a suitable light wavelength to maximize the imaging depth. Our results suggest that this technology could be a useful clinical tool, allowing clinicians to identify SLNs non-invasively in vivo. In the future, these contrast agents could be functionalized to do molecular photoacoustic imaging.",
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