In vivo reprogramming of wound-resident cells generates skin epithelial tissue

Masakazu Kurita, Toshikazu Araoka, Tomoaki Hishida, David D. O’keefe, Yuta Takahashi, Akihisa Sakamoto, Masahiro Sakurai, Keiichiro Suzuki, Jun Wu, Mako Yamamoto, Reyna Hernandez-Benitez, Alejandro Ocampo, Pradeep Reddy, Maxim Nikolaievich Shokhirev, Pierre Magistretti, Estrella Núñez Delicado, Hitomi Eto, Kiyonori Harii, Juan Carlos Izpisua Belmonte

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Large cutaneous ulcers are, in severe cases, life threatening1,2. As the global population ages, non-healing ulcers are becoming increasingly common1,2. Treatment currently requires the transplantation of pre-existing epithelial components, such as skin grafts, or therapy using cultured cells2. Here we develop alternative supplies of epidermal coverage for the treatment of these kinds of wounds. We generated expandable epithelial tissues using in vivo reprogramming of wound-resident mesenchymal cells. Transduction of four transcription factors that specify the skin-cell lineage enabled efficient and rapid de novo epithelialization from the surface of cutaneous ulcers in mice. Our findings may provide a new therapeutic avenue for treating skin wounds and could be extended to other disease situations in which tissue homeostasis and repair are impaired.

Original languageEnglish (US)
Pages (from-to)243-270
Number of pages28
JournalNature
Volume561
Issue number7722
DOIs
StatePublished - Jan 1 2018
Externally publishedYes

ASJC Scopus subject areas

  • General

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    Kurita, M., Araoka, T., Hishida, T., O’keefe, D. D., Takahashi, Y., Sakamoto, A., Sakurai, M., Suzuki, K., Wu, J., Yamamoto, M., Hernandez-Benitez, R., Ocampo, A., Reddy, P., Shokhirev, M. N., Magistretti, P., Delicado, E. N., Eto, H., Harii, K., & Belmonte, J. C. I. (2018). In vivo reprogramming of wound-resident cells generates skin epithelial tissue. Nature, 561(7722), 243-270. https://doi.org/10.1038/s41586-018-0477-4