In Vivo Single-Cell Detection of Metabolic Oscillations in Stem Cells

Chiara Stringari, Hong Wang, Mikhail Geyfman, Viera Crosignani, Vivek Kumar, Joseph S. Takahashi, Bogi Andersen, Enrico Gratton

Research output: Contribution to journalArticle

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Abstract

Through the use of bulk measurements in metabolic organs, the circadian clock was shown to play roles in organismal energy homeostasis. However, the relationship between metabolic and circadian oscillations has not been studied invivo at a single-cell level. Also, it is unknown whether the circadian clock controls metabolism in stem cells. We used a sensitive, noninvasive method to detect metabolic oscillations and circadian phase within epidermal stem cells in live mice at the single-cell level. We observe a higher NADH/NAD+ ratio, reflecting an increased glycolysis/oxidative phosphorylation ratio during the night compared to the day. Furthermore, we demonstrate that single-cell metabolic heterogeneity within the basal cell layer correlates with the circadian clock and that diurnal fluctuations in NADH/NAD+ ratio are Bmal1 dependent. Our data show that, in proliferating stem cells, the circadian clock coordinates activities of oxidative phosphorylation and glycolysis with DNA synthesis, perhaps as a protective mechanism against genotoxicity.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalCell Reports
Volume10
Issue number1
DOIs
StatePublished - Jan 6 2015

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Circadian Clocks
Stem cells
NAD
Clocks
Stem Cells
Oxidative Phosphorylation
Glycolysis
Metabolism
Homeostasis
Cells
DNA

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Stringari, C., Wang, H., Geyfman, M., Crosignani, V., Kumar, V., Takahashi, J. S., ... Gratton, E. (2015). In Vivo Single-Cell Detection of Metabolic Oscillations in Stem Cells. Cell Reports, 10(1), 1-7. https://doi.org/10.1016/j.celrep.2014.12.007

In Vivo Single-Cell Detection of Metabolic Oscillations in Stem Cells. / Stringari, Chiara; Wang, Hong; Geyfman, Mikhail; Crosignani, Viera; Kumar, Vivek; Takahashi, Joseph S.; Andersen, Bogi; Gratton, Enrico.

In: Cell Reports, Vol. 10, No. 1, 06.01.2015, p. 1-7.

Research output: Contribution to journalArticle

Stringari, C, Wang, H, Geyfman, M, Crosignani, V, Kumar, V, Takahashi, JS, Andersen, B & Gratton, E 2015, 'In Vivo Single-Cell Detection of Metabolic Oscillations in Stem Cells', Cell Reports, vol. 10, no. 1, pp. 1-7. https://doi.org/10.1016/j.celrep.2014.12.007
Stringari, Chiara ; Wang, Hong ; Geyfman, Mikhail ; Crosignani, Viera ; Kumar, Vivek ; Takahashi, Joseph S. ; Andersen, Bogi ; Gratton, Enrico. / In Vivo Single-Cell Detection of Metabolic Oscillations in Stem Cells. In: Cell Reports. 2015 ; Vol. 10, No. 1. pp. 1-7.
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