In vivo studies of cellular energy state, pH, and sodium in rat liver after thermal injury

Z. F. Xia, J. W. Horton, P. Y. Zhao, N. Bansal, E. E. Babcock, A. D. Sherry, C. R. Malloy

Research output: Contribution to journalArticle

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Abstract

In vivo 31P- and 23Na-magnetic resonance spectroscopy was used to measure phosphorus metabolites, intracellular pH, cytosolic free Mg2+, and intracellular Na+ in the liver of rats 24 h after 40% total body surface area full-thickness burn injury. Studies were performed during infusion of thulium (III) 1,4,7,10-tetraazacyclododecane N,N',N'',N'''- tetra(methylenephosphonate), which served as the Na+ shift agent. Compared with the sham-burn group, there was a significant increase in hepatic intracellular Na+ along with a decrease in intracellular pH and free Mg2+. The ratio of intra- to extracellular Na+ increased, indicating a decreased Na+ gradient that may determine the hepatic transmembrane potential difference. Hepatic β-ATP/P(i) also significantly decreased, which suggests that either ATP utilization is significantly accelerated or ATP synthesis is inhibited after the thermal injury. Of the cations measured (Na+, Mg2+, H+), the change in intracellular Na+ was most dramatic. This study demonstrates that major burn injury may cause profound changes in hepatic bioenergetics and ionic metabolism 24 h after injury and that intracellular Na+ may be a sensitive indicator of hepatic dysfunction 24 h after injury. Because these animals tolerated the shift reagent, thulium (III) 1,4,7,10- tetraazacyclododecane N,N',N'',N'''-tetra(methylenephosphonate), nuclear magnetic resonance spectroscopy may prove valuable in monitoring intracellular cations in the liver after major injury.

Original languageEnglish (US)
Pages (from-to)1507-1511
Number of pages5
JournalJournal of applied physiology
Volume76
Issue number4
DOIs
StatePublished - Jan 1 1994

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Keywords

  • burn injury
  • energetics
  • high-energy phosphates

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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