In vivo transcripts of the S-layer-encoding structural gene of the archaeon Methanococcus voltae

Janice W. Kansy, Maria E. Carinato, Lisa M Monteggia, Jordan Konisky

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The 5′ region of the S-layer-encoding structural gene (sla) of Methanococcus voltae was sequenced. The sequence information was then used to identify the in vivo transcription products of the gene. We observed three transcripts, and upstream from each transcription start point was a region with similarity to the Box A consensus sequence observed in archaeal promoters. In two of the three cases, two Box A sequences were present in tandem. This arrangement may play a role in the high level of gene expression expected for the sla gene. Presumptive archaeal Box B signatures were also identified.

Original languageEnglish (US)
Pages (from-to)131-135
Number of pages5
JournalGene
Volume148
Issue number1
DOIs
StatePublished - Oct 11 1994

Keywords

  • DNA sequence
  • primer extension
  • promoters
  • transcription initiation

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'In vivo transcripts of the S-layer-encoding structural gene of the archaeon Methanococcus voltae'. Together they form a unique fingerprint.

  • Cite this