Incertohypothalamic A13 dopamine neurons

Effect of gonadal steroids on tyrosine hydroxylase

M. K. Sanghera, S. Grady, W. Smith, D. J. Woodward, J. C. Porter

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this study the effect of gonadectomy and steroid treatment on the dorsal component of the incertohypothalamic dopamine system or nucleus A13 was assessed by immunocytochemistry using an antibody raised to tyrosine hydroxylase (TH). A computer graphic system interfaced to a microscope was used to count and measure the diameters of TH positive neurons and display the data in the three-dimensional space of the nucleus. In males, castration resulted in a dramatic decrease in the reaction product representative of TH. The number of TH-positive cells in the A13 DA nucleus decreased to 25% of intact levels. In females, ovariectomy also caused an impressive loss in the TH-immunostainable material, but this was not indicated by a change in the total number of TH-positive neurons. In both sexes the loss in TH immunostain was confined mainly to the mid-portion of the nucleus. Hormone treatment restored the TH immunostain (cell number and size) to and/or above intact levels in both sexes. These data suggest that A13 TH immunostain is stimulated by gonadal steroids in male and female rats.

Original languageEnglish (US)
Pages (from-to)268-275
Number of pages8
JournalNeuroendocrinology
Volume53
Issue number3
StatePublished - 1991

Fingerprint

Dopamine Agents
Dopaminergic Neurons
Tyrosine 3-Monooxygenase
Steroids
Computer Graphics
Data Display
Neurons
Orchiectomy
Computer Systems
Ovariectomy
Cell Size
Dopamine
Cell Count
Immunohistochemistry
Hormones
Antibodies

Keywords

  • computer graphic system
  • estrogen
  • progesterone
  • testosterone

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)

Cite this

Sanghera, M. K., Grady, S., Smith, W., Woodward, D. J., & Porter, J. C. (1991). Incertohypothalamic A13 dopamine neurons: Effect of gonadal steroids on tyrosine hydroxylase. Neuroendocrinology, 53(3), 268-275.

Incertohypothalamic A13 dopamine neurons : Effect of gonadal steroids on tyrosine hydroxylase. / Sanghera, M. K.; Grady, S.; Smith, W.; Woodward, D. J.; Porter, J. C.

In: Neuroendocrinology, Vol. 53, No. 3, 1991, p. 268-275.

Research output: Contribution to journalArticle

Sanghera, MK, Grady, S, Smith, W, Woodward, DJ & Porter, JC 1991, 'Incertohypothalamic A13 dopamine neurons: Effect of gonadal steroids on tyrosine hydroxylase', Neuroendocrinology, vol. 53, no. 3, pp. 268-275.
Sanghera MK, Grady S, Smith W, Woodward DJ, Porter JC. Incertohypothalamic A13 dopamine neurons: Effect of gonadal steroids on tyrosine hydroxylase. Neuroendocrinology. 1991;53(3):268-275.
Sanghera, M. K. ; Grady, S. ; Smith, W. ; Woodward, D. J. ; Porter, J. C. / Incertohypothalamic A13 dopamine neurons : Effect of gonadal steroids on tyrosine hydroxylase. In: Neuroendocrinology. 1991 ; Vol. 53, No. 3. pp. 268-275.
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