Incorporation of hypoxanthine by PHA stimulated, HPRT deficient lymphocytes

R. P. Cox, M. R. Krauss, M. E. Balis

Research output: Contribution to journalArticle

Abstract

Phytohemagglutinin (PHA) markedly stimulates 3H hypoxanthine incorporation by lymphocytes of normal subjects as revealed by radioautography. There is no corresponding increase in activity of hypoxanthine phosphoribosyl transferase (HPRT) in lysates, but the level of phosphoribosyl pyrophosphate (PRPP), the cosubstrate for the reaction, is higher. Lymphocytes from a patient with a partial HPRT deficiency responded to PHA as did the normal subjects, whereas the response in subjects with Lesch Nyhan syndrome (LN) was variable. PHA stimulated lymphocytes from two patients with LN showed some increase in 3H hypoxanthine incorporation, while two others failed to respond. The observations provide further evidence of genetic heterogeneity among LN patients.

Original languageEnglish (US)
Number of pages1
JournalBulletin of the New York Academy of Medicine: Journal of Urban Health
Volume51
Issue number10
StatePublished - Jan 1 1975

    Fingerprint

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this