Phytohemagglutinin (PHA) markedly stimulates 3H hypoxanthine incorporation by lymphocytes of normal subjects as revealed by radioautography. There is no corresponding increase in activity of hypoxanthine phosphoribosyl transferase (HPRT) in lysates, but the level of phosphoribosyl pyrophosphate (PRPP), the cosubstrate for the reaction, is higher. Lymphocytes from a patient with a partial HPRT deficiency responded to PHA as did the normal subjects, whereas the response in subjects with Lesch Nyhan syndrome (LN) was variable. PHA stimulated lymphocytes from two patients with LN showed some increase in 3H hypoxanthine incorporation, while two others failed to respond. The observations provide further evidence of genetic heterogeneity among LN patients.
|Original language||English (US)|
|Number of pages||1|
|Journal||Bulletin of the New York Academy of Medicine: Journal of Urban Health|
|State||Published - Jan 1 1975|
ASJC Scopus subject areas
- Public Health, Environmental and Occupational Health