Incorporation of hypoxanthine by PHA stimulated, HPRT deficient lymphocytes

R. P. Cox; M. R. Krauss; M. E. Balis

Incorporation of hypoxanthine by PHA stimulated, HPRT deficient lymphocytes

R. P. Cox, M. R. Krauss, M. E. Balis

Research output: Contribution to journal › Article › peer-review

Abstract

Phytohemagglutinin (PHA) markedly stimulates ³H hypoxanthine incorporation by lymphocytes of normal subjects as revealed by radioautography. There is no corresponding increase in activity of hypoxanthine phosphoribosyl transferase (HPRT) in lysates, but the level of phosphoribosyl pyrophosphate (PRPP), the cosubstrate for the reaction, is higher. Lymphocytes from a patient with a partial HPRT deficiency responded to PHA as did the normal subjects, whereas the response in subjects with Lesch Nyhan syndrome (LN) was variable. PHA stimulated lymphocytes from two patients with LN showed some increase in ³H hypoxanthine incorporation, while two others failed to respond. The observations provide further evidence of genetic heterogeneity among LN patients.

Original language	English (US)
Pages (from-to)	1153
Number of pages	1
Journal	Bulletin of the New York Academy of Medicine: Journal of Urban Health
Volume	51
Issue number	10
State	Published - 1975

ASJC Scopus subject areas

Public Health, Environmental and Occupational Health

Cite this

@article{70db29520f8f4a1ea674d58ef5675d60,

title = "Incorporation of hypoxanthine by PHA stimulated, HPRT deficient lymphocytes",

abstract = "Phytohemagglutinin (PHA) markedly stimulates 3H hypoxanthine incorporation by lymphocytes of normal subjects as revealed by radioautography. There is no corresponding increase in activity of hypoxanthine phosphoribosyl transferase (HPRT) in lysates, but the level of phosphoribosyl pyrophosphate (PRPP), the cosubstrate for the reaction, is higher. Lymphocytes from a patient with a partial HPRT deficiency responded to PHA as did the normal subjects, whereas the response in subjects with Lesch Nyhan syndrome (LN) was variable. PHA stimulated lymphocytes from two patients with LN showed some increase in 3H hypoxanthine incorporation, while two others failed to respond. The observations provide further evidence of genetic heterogeneity among LN patients.",

author = "Cox, {R. P.} and Krauss, {M. R.} and Balis, {M. E.}",

note = "Funding Information: This investigation was supported by USPHS grants AM 14528,C A 08748a nd HD 04526a nd National Foundation CB-232. R. P. C. is a Career Scientisto f the Health ResearchC ouncil of the City of New York and J. D. is a Career Investigator of the National Instituteso f Child Health and Human Development.",

year = "1975",

language = "English (US)",

volume = "51",

pages = "1153",

journal = "Bulletin of the New York Academy of Medicine: Journal of Urban Health",

issn = "0028-7091",

publisher = "Springer Science and Business Media Deutschland GmbH",

number = "10",

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TY - JOUR

T1 - Incorporation of hypoxanthine by PHA stimulated, HPRT deficient lymphocytes

AU - Cox, R. P.

AU - Krauss, M. R.

AU - Balis, M. E.

N1 - Funding Information: This investigation was supported by USPHS grants AM 14528,C A 08748a nd HD 04526a nd National Foundation CB-232. R. P. C. is a Career Scientisto f the Health ResearchC ouncil of the City of New York and J. D. is a Career Investigator of the National Instituteso f Child Health and Human Development.

PY - 1975

Y1 - 1975

N2 - Phytohemagglutinin (PHA) markedly stimulates 3H hypoxanthine incorporation by lymphocytes of normal subjects as revealed by radioautography. There is no corresponding increase in activity of hypoxanthine phosphoribosyl transferase (HPRT) in lysates, but the level of phosphoribosyl pyrophosphate (PRPP), the cosubstrate for the reaction, is higher. Lymphocytes from a patient with a partial HPRT deficiency responded to PHA as did the normal subjects, whereas the response in subjects with Lesch Nyhan syndrome (LN) was variable. PHA stimulated lymphocytes from two patients with LN showed some increase in 3H hypoxanthine incorporation, while two others failed to respond. The observations provide further evidence of genetic heterogeneity among LN patients.

AB - Phytohemagglutinin (PHA) markedly stimulates 3H hypoxanthine incorporation by lymphocytes of normal subjects as revealed by radioautography. There is no corresponding increase in activity of hypoxanthine phosphoribosyl transferase (HPRT) in lysates, but the level of phosphoribosyl pyrophosphate (PRPP), the cosubstrate for the reaction, is higher. Lymphocytes from a patient with a partial HPRT deficiency responded to PHA as did the normal subjects, whereas the response in subjects with Lesch Nyhan syndrome (LN) was variable. PHA stimulated lymphocytes from two patients with LN showed some increase in 3H hypoxanthine incorporation, while two others failed to respond. The observations provide further evidence of genetic heterogeneity among LN patients.

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JO - Bulletin of the New York Academy of Medicine: Journal of Urban Health

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IS - 10

ER -

Incorporation of hypoxanthine by PHA stimulated, HPRT deficient lymphocytes

Abstract

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