Incorporation of hypoxanthine by PHA stimulated, HPRT deficient lymphocytes

R. P. Cox, M. R. Krauss, M. E. Balis

Research output: Contribution to journalArticle

Abstract

Phytohemagglutinin (PHA) markedly stimulates 3H hypoxanthine incorporation by lymphocytes of normal subjects as revealed by radioautography. There is no corresponding increase in activity of hypoxanthine phosphoribosyl transferase (HPRT) in lysates, but the level of phosphoribosyl pyrophosphate (PRPP), the cosubstrate for the reaction, is higher. Lymphocytes from a patient with a partial HPRT deficiency responded to PHA as did the normal subjects, whereas the response in subjects with Lesch Nyhan syndrome (LN) was variable. PHA stimulated lymphocytes from two patients with LN showed some increase in 3H hypoxanthine incorporation, while two others failed to respond. The observations provide further evidence of genetic heterogeneity among LN patients.

Original languageEnglish (US)
Pages (from-to)1153
Number of pages1
JournalBulletin of the New York Academy of Medicine: Journal of Urban Health
Volume51
Issue number10
StatePublished - 1975

Fingerprint

Hypoxanthine
Lesch-Nyhan Syndrome
Phytohemagglutinins
Transferases
Lymphocytes
Phosphoribosyl Pyrophosphate
Genetic Heterogeneity
Autoradiography
evidence

ASJC Scopus subject areas

  • Public Health, Environmental and Occupational Health

Cite this

Incorporation of hypoxanthine by PHA stimulated, HPRT deficient lymphocytes. / Cox, R. P.; Krauss, M. R.; Balis, M. E.

In: Bulletin of the New York Academy of Medicine: Journal of Urban Health, Vol. 51, No. 10, 1975, p. 1153.

Research output: Contribution to journalArticle

@article{70db29520f8f4a1ea674d58ef5675d60,
title = "Incorporation of hypoxanthine by PHA stimulated, HPRT deficient lymphocytes",
abstract = "Phytohemagglutinin (PHA) markedly stimulates 3H hypoxanthine incorporation by lymphocytes of normal subjects as revealed by radioautography. There is no corresponding increase in activity of hypoxanthine phosphoribosyl transferase (HPRT) in lysates, but the level of phosphoribosyl pyrophosphate (PRPP), the cosubstrate for the reaction, is higher. Lymphocytes from a patient with a partial HPRT deficiency responded to PHA as did the normal subjects, whereas the response in subjects with Lesch Nyhan syndrome (LN) was variable. PHA stimulated lymphocytes from two patients with LN showed some increase in 3H hypoxanthine incorporation, while two others failed to respond. The observations provide further evidence of genetic heterogeneity among LN patients.",
author = "Cox, {R. P.} and Krauss, {M. R.} and Balis, {M. E.}",
year = "1975",
language = "English (US)",
volume = "51",
pages = "1153",
journal = "Journal of Urban Health",
issn = "1099-3460",
publisher = "Springer Science and Business Media Deutschland GmbH",
number = "10",

}

TY - JOUR

T1 - Incorporation of hypoxanthine by PHA stimulated, HPRT deficient lymphocytes

AU - Cox, R. P.

AU - Krauss, M. R.

AU - Balis, M. E.

PY - 1975

Y1 - 1975

N2 - Phytohemagglutinin (PHA) markedly stimulates 3H hypoxanthine incorporation by lymphocytes of normal subjects as revealed by radioautography. There is no corresponding increase in activity of hypoxanthine phosphoribosyl transferase (HPRT) in lysates, but the level of phosphoribosyl pyrophosphate (PRPP), the cosubstrate for the reaction, is higher. Lymphocytes from a patient with a partial HPRT deficiency responded to PHA as did the normal subjects, whereas the response in subjects with Lesch Nyhan syndrome (LN) was variable. PHA stimulated lymphocytes from two patients with LN showed some increase in 3H hypoxanthine incorporation, while two others failed to respond. The observations provide further evidence of genetic heterogeneity among LN patients.

AB - Phytohemagglutinin (PHA) markedly stimulates 3H hypoxanthine incorporation by lymphocytes of normal subjects as revealed by radioautography. There is no corresponding increase in activity of hypoxanthine phosphoribosyl transferase (HPRT) in lysates, but the level of phosphoribosyl pyrophosphate (PRPP), the cosubstrate for the reaction, is higher. Lymphocytes from a patient with a partial HPRT deficiency responded to PHA as did the normal subjects, whereas the response in subjects with Lesch Nyhan syndrome (LN) was variable. PHA stimulated lymphocytes from two patients with LN showed some increase in 3H hypoxanthine incorporation, while two others failed to respond. The observations provide further evidence of genetic heterogeneity among LN patients.

UR - http://www.scopus.com/inward/record.url?scp=0016695124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016695124&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0016695124

VL - 51

SP - 1153

JO - Journal of Urban Health

JF - Journal of Urban Health

SN - 1099-3460

IS - 10

ER -