TY - JOUR
T1 - Increased c-fos mRNA expression by human fibroblasts contracting stressed collagen matrices
AU - Rosenfeldt, Hans
AU - Lee, David J.
AU - Grinnell, Frederick
PY - 1998/5
Y1 - 1998/5
N2 - We studied early changes in gene expression during fibroblast contraction of stressed collagen matrices. The level of c-fos mRNA increased dramatically and peaked 50 to 60 min after matrix contraction was initiated. This response did not require serum and could not be accounted for simply by disruption of the actin cytoskeleton. Increased c-fos mRNA levels required Ca2+ influx but not the cyclic AMP or extracellular signal-regulated kinase (ERK 1/2) signaling pathways, both of which are activated when fibroblasts contract stressed collagen matrices. The levels of two other immediate-early genes, fosb and c-jun, also increased transiently after fibroblast contraction, whereas the levels of fra-1,fra-2, c-myc, and the transcription factor NF-κB remained the same, indicating that fibroblast contraction caused changes in a selective group of genes. The increase in c-fos mRNA during contraction of stressed collagen matrices may reflect a unique role for c-fos in mechanoregulated events at the end of wound repair.
AB - We studied early changes in gene expression during fibroblast contraction of stressed collagen matrices. The level of c-fos mRNA increased dramatically and peaked 50 to 60 min after matrix contraction was initiated. This response did not require serum and could not be accounted for simply by disruption of the actin cytoskeleton. Increased c-fos mRNA levels required Ca2+ influx but not the cyclic AMP or extracellular signal-regulated kinase (ERK 1/2) signaling pathways, both of which are activated when fibroblasts contract stressed collagen matrices. The levels of two other immediate-early genes, fosb and c-jun, also increased transiently after fibroblast contraction, whereas the levels of fra-1,fra-2, c-myc, and the transcription factor NF-κB remained the same, indicating that fibroblast contraction caused changes in a selective group of genes. The increase in c-fos mRNA during contraction of stressed collagen matrices may reflect a unique role for c-fos in mechanoregulated events at the end of wound repair.
UR - http://www.scopus.com/inward/record.url?scp=0031893013&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031893013&partnerID=8YFLogxK
U2 - 10.1128/MCB.18.5.2659
DO - 10.1128/MCB.18.5.2659
M3 - Article
C2 - 9566885
AN - SCOPUS:0031893013
SN - 0270-7306
VL - 18
SP - 2659
EP - 2667
JO - Molecular and cellular biology
JF - Molecular and cellular biology
IS - 5
ER -