Increased ER-mitochondrial coupling promotes mitochondrial respiration and bioenergetics during early phases of ER stress

Roberto Bravo, Jose Miguel Vicencio, Valentina Parra, Rodrigo Troncoso, Juan Pablo Munoz, Michael Bui, Clara Quiroga, Andrea E. Rodriguez, Hugo E. Verdejo, Jorge Ferreira, Myriam Iglewski, Mario Chiong, Thomas Simmen, Antonio Zorzano, Joseph A Hill, Beverly A Rothermel, Gyorgy Szabadkai, Sergio Lavandero

Research output: Contribution to journalComment/debate

239 Scopus citations

Abstract

Increasing evidence indicates that endoplasmic reticulum (ER) stress activates the adaptive unfolded protein response (UPR), but that beyond a certain degree of ER damage, this response triggers apoptotic pathways. The general mechanisms of the UPR and its apoptotic pathways are well characterized. However, the metabolic events that occur during the adaptive phase of ER stress, before the cell death response, remain unknown. Here, we show that, during the onset of ER stress, the reticular and mitochondrial networks are redistributed towards the perinuclear area and their points of connection are increased in a microtubule-dependent fashion. A localized increase in mitochondrial transmembrane potential is observed only in redistributed mitochondria, whereas mitochondria that remain in other subcellular zones display no significant changes. Spatial re-organization of these organelles correlates with an increase in ATP levels, oxygen consumption, reductive power and increased mitochondrial Ca 2+ uptake. Accordingly, uncoupling of the organelles or blocking Ca 2+ transfer impaired the metabolic response, rendering cells more vulnerable to ER stress. Overall, these data indicate that ER stress induces an early increase in mitochondrial metabolism that depends crucially upon organelle coupling and Ca 2+ transfer, which, by enhancing cellular bioenergetics, establishes the metabolic basis for the adaptation to this response.

Original languageEnglish (US)
Pages (from-to)2143-2152
Number of pages10
JournalJournal of cell science
Volume124
Issue number13
DOIs
StatePublished - Jul 1 2011

Keywords

  • Ca
  • Endoplasmic reticulum stress
  • Metabolism
  • Mitochondria
  • Mitofusin 2 (Mfn2)
  • Unfolded protein response (UPR)

ASJC Scopus subject areas

  • Cell Biology

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  • Cite this

    Bravo, R., Vicencio, J. M., Parra, V., Troncoso, R., Munoz, J. P., Bui, M., Quiroga, C., Rodriguez, A. E., Verdejo, H. E., Ferreira, J., Iglewski, M., Chiong, M., Simmen, T., Zorzano, A., Hill, J. A., Rothermel, B. A., Szabadkai, G., & Lavandero, S. (2011). Increased ER-mitochondrial coupling promotes mitochondrial respiration and bioenergetics during early phases of ER stress. Journal of cell science, 124(13), 2143-2152. https://doi.org/10.1242/jcs.080762