Increased expression of an endopeptidase (γ-EGE/IDE) hydrolyzing β- endorphin during differentiation and maturation of bone marrow macrophages

Boppana Sarada, Dwain L Thiele, Tuyet Dang, Afshin Safavi, Louis B. Hersh, Gene L. Cottam

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The presence and regulated expression of peptidase activity is a powerful mechanism with the potential to terminate or alter receptor recognition, cell membrane signal transduction, and physiological responses of immune cells to exogenous opioid peptides. In this study, the expression of an endopeptidase that hydrolyzes β-endorphin to γ-endorphin and other peptide products was investigated during in vitro differentiation and maturation of recombinant granulocyte-macrophage colony-stimulating factor (rGM-CSF) -derived, bone marrow-derived macrophages. In freshly isolated intact isolated mouse bone marrow cells the rate of β-endorphin hydrolysis is undetectable (<0.1 nmol β-endorphin hydrolyzed/h/106 cells). However, total intracellular β-endorphin hydrolytic activity was increased significantly to 20.0 ± 1.7 nmol/h/106 cells in the mature mouse macrophages derived in vitro by culture with rGM-CSF. rGM-CSF-derived macrophages expressed significantly higher levels of both protein and mRNA for the major β-endorphin endopeptidase, γ-endorphin-generating enzyme/insulin-degrading enzyme (γ-EGE/IDE). Moreover, this enzymatic activity appears to be responsible for cleavage of exogenous β-endorphin by intact rGM-CSF-derived macrophages or peritoneal macrophages to generate γ- endorphin and other peptide products.

Original languageEnglish (US)
Pages (from-to)753-760
Number of pages8
JournalJournal of Leukocyte Biology
Volume62
Issue number6
StatePublished - Dec 1997

Fingerprint

Endorphins
Endopeptidases
Bone Marrow
Macrophages
Granulocyte-Macrophage Colony-Stimulating Factor
Insulysin
Peptides
Opioid Peptides
Peritoneal Macrophages
Bone Marrow Cells
Signal Transduction
Hydrolysis
Peptide Hydrolases
Cell Membrane
Messenger RNA

Keywords

  • Bone marrow cell
  • Mouse
  • rGM-CSF

ASJC Scopus subject areas

  • Cell Biology

Cite this

Increased expression of an endopeptidase (γ-EGE/IDE) hydrolyzing β- endorphin during differentiation and maturation of bone marrow macrophages. / Sarada, Boppana; Thiele, Dwain L; Dang, Tuyet; Safavi, Afshin; Hersh, Louis B.; Cottam, Gene L.

In: Journal of Leukocyte Biology, Vol. 62, No. 6, 12.1997, p. 753-760.

Research output: Contribution to journalArticle

Sarada, Boppana ; Thiele, Dwain L ; Dang, Tuyet ; Safavi, Afshin ; Hersh, Louis B. ; Cottam, Gene L. / Increased expression of an endopeptidase (γ-EGE/IDE) hydrolyzing β- endorphin during differentiation and maturation of bone marrow macrophages. In: Journal of Leukocyte Biology. 1997 ; Vol. 62, No. 6. pp. 753-760.
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