Increased expression of lectinlike oxidized low density lipoprotein receptor-1 in initial atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits

Mingyi Chen, Makoto Kakutani, Manabu Minami, Hiroharu Kataoka, Noriaki Kume, Shuh Narumiya, Toru Kita, Tomoh Masaki, Tatsuya Sawamura

Research output: Contribution to journalArticle

163 Scopus citations

Abstract

A novel lectinlike oxidized low density lipoprotein receptor-1 (LOX-1) was recently identified in bovine aortic endothelial cells. It is strongly suggested to have a potential role in the initiation and development of atherosclerosis. In this study, we have isolated cDNA clones encoding the rabbit homologue of LOX-1 by screening a rabbit placenta cDNA library. In amino acid sequence and domain structure organization, the rabbit LOX-1 is highly conserved with the human counterpart. Transfection of rabbit LOX-1 cDNA to HEK-293 cells confers on them the activity to bind and internalize oxidized low density lipoprotein. Rabbit LOX-1 was identified as a 45-kDa protein by Western blot analysis with a specific monoclonal antibody. Notably, analyses by reverse transcription-polymerase chain reaction and Western blot revealed that LOX-1 was accumulated in 8-week-old Watanabe heritable hyperlipidemic rabbit aortas compared with normal rabbit aortas. Immunostaining confirmed that the augmented expression of LOX-1 was primarily localized within the intima at the earliest stages of atherogenesis. The most prominent staining was in the endothelial cells of lesions. Furthermore, the distinctive staining of LOX-1 was identified in the endothelium of nonlesion areas of Watanabe heritable hyperlipidemic rabbit aortas. Taken together, these findings support the possibility that LOX-1 might be involved in the initiation of atherosclerosis.

Original languageEnglish (US)
Pages (from-to)1107-1115
Number of pages9
JournalArteriosclerosis, thrombosis, and vascular biology
Volume20
Issue number4
DOIs
StatePublished - Apr 2000

Keywords

  • Atherosclerosis
  • Endothelium
  • Lectinlike oxidized low density lipoprotein receptor-1
  • Oxidized low density lipoproteins
  • Watanabe heritable hyperlipidemic rabbits

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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