Increased expression of mutated Ha-ras during premalignant progression in SENCAR mouse skin

Marcelo L. Rodriguez-Puebla, Margaret LaCava, Marcela F. Bolontrade, Jamie Russell, Claudio J. Conti

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The ras proto-oncogene family products are membrane-associated, guanine nucleotide-binding proteins that serve as a molecular switch for signal transduction pathways in a diverse array of organisms. In the mouse skin two- stage carcinogenesis model, a specific point mutation in Ha-ras codon 61 is responsible for the initiation event. Here we investigated whether Ha-ras protein and mRNA expression change during premalignant progression. Also, we assessed the Ha-ras mutated allele after these changes. To those ends, we analysed the Ha-ras expression profiles in normal and hyperplastic skin, papillomas, and squamous cell carcinomas by western blotting, reverse transcription-polymerase chain reaction, and in situ hybridization. Increased levels of Ha-ras expression were observed at specific times during promotion. These changes were followed by an increase in the level of expression of the Ha-ras mutated allele. These results suggest that increased expression of Ha- ras mutated alleles may have an important role during premalignant progression.

Original languageEnglish (US)
Pages (from-to)150-156
Number of pages7
JournalMolecular Carcinogenesis
Volume26
Issue number3
DOIs
StatePublished - Nov 22 1999

Fingerprint

Inbred SENCAR Mouse
Alleles
Skin
ras Proteins
Guanine Nucleotides
ras Genes
Proto-Oncogenes
Oncogene Proteins
Papilloma
Point Mutation
Codon
Reverse Transcription
In Situ Hybridization
Squamous Cell Carcinoma
Signal Transduction
Carrier Proteins
Carcinogenesis
Western Blotting
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Papilloma
  • Ras
  • Squamous cell carcinoma
  • Tumor progression
  • Two-stage carcinogenesis

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

Cite this

Increased expression of mutated Ha-ras during premalignant progression in SENCAR mouse skin. / Rodriguez-Puebla, Marcelo L.; LaCava, Margaret; Bolontrade, Marcela F.; Russell, Jamie; Conti, Claudio J.

In: Molecular Carcinogenesis, Vol. 26, No. 3, 22.11.1999, p. 150-156.

Research output: Contribution to journalArticle

Rodriguez-Puebla, Marcelo L. ; LaCava, Margaret ; Bolontrade, Marcela F. ; Russell, Jamie ; Conti, Claudio J. / Increased expression of mutated Ha-ras during premalignant progression in SENCAR mouse skin. In: Molecular Carcinogenesis. 1999 ; Vol. 26, No. 3. pp. 150-156.
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