Increased mRNA for low density lipoprotein receptor in livers of rabbits treated with 17α-ethinyl estradiol

P. T S Ma; T. Yamamoto; J. L. Goldstein; M. S. Brown

doi:10.1073/pnas.83.3.792

Increased mRNA for low density lipoprotein receptor in livers of rabbits treated with 17α-ethinyl estradiol

P. T S Ma, T. Yamamoto, J. L. Goldstein, M. S. Brown

Research output: Contribution to journal › Article › peer-review

177 Scopus citations

Abstract

Pharmacologic doses of 17α-ethinyl estradiol are known to increase the number of low density lipoprotein (LDL) receptors in livers of rats, thereby producing a profound fall in plasma cholesterol levels. We now report that ethinyl estradiol exerts the same effect in livers of male and female rabbits and that the increase in receptor number is correlated with a 6- to 8-fold increase in the levels of receptor mRNA. Receptor protein was measured by ligand blotting, and mRNA levels were measured by a quantitative solution hybridization/S1 nuclease protection assay using uniformly ³²P-labeled single-stranded cDNA probes. These experiments demonstrate that pharmacologic induction of the mRNA for the LDL receptor in liver can lead to increased LDL receptor levels and a fall in plasma cholesterol in experimental animals.

Original language	English (US)
Pages (from-to)	792-796
Number of pages	5
Journal	Proceedings of the National Academy of Sciences of the United States of America
Volume	83
Issue number	3
DOIs	https://doi.org/10.1073/pnas.83.3.792
State	Published - 1986

ASJC Scopus subject areas

General

Access to Document

10.1073/pnas.83.3.792

Cite this

@article{31753b4ac5ba48648482eb82a789e4d3,

title = "Increased mRNA for low density lipoprotein receptor in livers of rabbits treated with 17α-ethinyl estradiol",

abstract = "Pharmacologic doses of 17α-ethinyl estradiol are known to increase the number of low density lipoprotein (LDL) receptors in livers of rats, thereby producing a profound fall in plasma cholesterol levels. We now report that ethinyl estradiol exerts the same effect in livers of male and female rabbits and that the increase in receptor number is correlated with a 6- to 8-fold increase in the levels of receptor mRNA. Receptor protein was measured by ligand blotting, and mRNA levels were measured by a quantitative solution hybridization/S1 nuclease protection assay using uniformly 32P-labeled single-stranded cDNA probes. These experiments demonstrate that pharmacologic induction of the mRNA for the LDL receptor in liver can lead to increased LDL receptor levels and a fall in plasma cholesterol in experimental animals.",

author = "Ma, {P. T S} and T. Yamamoto and Goldstein, {J. L.} and Brown, {M. S.}",

year = "1986",

doi = "10.1073/pnas.83.3.792",

language = "English (US)",

volume = "83",

pages = "792--796",

journal = "Proceedings of the National Academy of Sciences of the United States of America",

issn = "0027-8424",

publisher = "National Academy of Sciences",

number = "3",

}

TY - JOUR

T1 - Increased mRNA for low density lipoprotein receptor in livers of rabbits treated with 17α-ethinyl estradiol

AU - Ma, P. T S

AU - Yamamoto, T.

AU - Goldstein, J. L.

AU - Brown, M. S.

PY - 1986

Y1 - 1986

N2 - Pharmacologic doses of 17α-ethinyl estradiol are known to increase the number of low density lipoprotein (LDL) receptors in livers of rats, thereby producing a profound fall in plasma cholesterol levels. We now report that ethinyl estradiol exerts the same effect in livers of male and female rabbits and that the increase in receptor number is correlated with a 6- to 8-fold increase in the levels of receptor mRNA. Receptor protein was measured by ligand blotting, and mRNA levels were measured by a quantitative solution hybridization/S1 nuclease protection assay using uniformly 32P-labeled single-stranded cDNA probes. These experiments demonstrate that pharmacologic induction of the mRNA for the LDL receptor in liver can lead to increased LDL receptor levels and a fall in plasma cholesterol in experimental animals.

AB - Pharmacologic doses of 17α-ethinyl estradiol are known to increase the number of low density lipoprotein (LDL) receptors in livers of rats, thereby producing a profound fall in plasma cholesterol levels. We now report that ethinyl estradiol exerts the same effect in livers of male and female rabbits and that the increase in receptor number is correlated with a 6- to 8-fold increase in the levels of receptor mRNA. Receptor protein was measured by ligand blotting, and mRNA levels were measured by a quantitative solution hybridization/S1 nuclease protection assay using uniformly 32P-labeled single-stranded cDNA probes. These experiments demonstrate that pharmacologic induction of the mRNA for the LDL receptor in liver can lead to increased LDL receptor levels and a fall in plasma cholesterol in experimental animals.

UR - http://www.scopus.com/inward/record.url?scp=0022456499&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022456499&partnerID=8YFLogxK

U2 - 10.1073/pnas.83.3.792

DO - 10.1073/pnas.83.3.792

M3 - Article

C2 - 2418443

AN - SCOPUS:0022456499

SN - 0027-8424

VL - 83

SP - 792

EP - 796

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

IS - 3

ER -

Increased mRNA for low density lipoprotein receptor in livers of rabbits treated with 17α-ethinyl estradiol

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this