Increased recovery of brain acetylcholinesterase activity in dichlorvos-intoxicated European eels Anguilla anguilla by bath treatment with N-acetylcysteine

Samuel Peña-Llopis, Ma Dolores Ferrando, Juan B. Peña

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Organophosphate (OP) pesticides are widely used as antiparasitic chemicals in finfish aquaculture. However, current antidotes cannot be applied to treat intoxicated fish. We showed in previous studies the importance of glutathione (GSH) metabolism in pesticide resistance of the European eel Anguilla anguilla L. The present work studied the effects of the antioxidant and glutathione pro-drug N-acetyl-L-cysteine (NAC) on the recovery of European eels exposed for 96 h to a sublethal concentration (0.17 mg l-1; 20% of its 96 h LC50) of the OP pesticide dichlorvos (2,2-dichlorovinyl dimethyl phosphate; DDVP). This insecticide and acaricide decreased muscular GSH content and increased oxidised glutathione (GSSG), lowering the GSH:GSSG ratio, which is indicative of a condition of oxidative stress. Acetylcholinesterase (ACHE) and glutathione reductase (GR) activities in the brain, which were biomarkers of neurotoxicity and oxidative stress, respectively, were also highly inhibited. Recovery in a 0.5 mM (81.6 mg l-1) NAC concentration ameliorated muscular GSH depletion, GSH:GSSG ratio, and the inhibition of brain AChE and GR activities. Hence, this is the first evidence of improved recovery of organophosphate-poisoned fish by bath treatments.

Original languageEnglish (US)
Pages (from-to)237-245
Number of pages9
JournalDiseases of Aquatic Organisms
Volume55
Issue number3
StatePublished - Aug 4 2003

Fingerprint

dichlorvos
acetylcysteine
Anguilla anguilla
acetylcholinesterase
eel
brain
glutathione
organophosphorus pesticides
glutathione-disulfide reductase
oxidative stress
pesticide resistance
acaricide
antidotes
finfish
neurotoxicity
acaricides
organophosphate
organophosphorus compounds
fish culture
fish

Keywords

  • AChE
  • Cholinesterase inhibitor
  • Detoxification
  • Glutathione metabolism
  • N-acetyl-L-cysteine
  • Organophosphate poisoning
  • Organophosphorus pesticide
  • Oxidative stress

ASJC Scopus subject areas

  • Aquatic Science
  • Ecology

Cite this

Increased recovery of brain acetylcholinesterase activity in dichlorvos-intoxicated European eels Anguilla anguilla by bath treatment with N-acetylcysteine. / Peña-Llopis, Samuel; Dolores Ferrando, Ma; Peña, Juan B.

In: Diseases of Aquatic Organisms, Vol. 55, No. 3, 04.08.2003, p. 237-245.

Research output: Contribution to journalArticle

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