Increased release of δ-aminolevulinic acid from protein during inhibition of protein synthesis in heart: Evidence for the extensive reutilization of heme in cardiac protein metabolism

John M. Ord, Kern Wildenthal

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Inhibition of protein synthesis with cycloheximide or puromycin caused a decrease in total cardiac proteolysis, as measured by the rate of release of radioactive phenylalanine from protein. At the same time, the rate of release of δ-aminolevulinic acid (δALA), which is incorporated specifically into heme-containing proteins, was increased significantly. These results suggest that, contrary to previously accepted beliefs, heme is extensively recycled in cardiac protein synthesis; therefore, the release of δALA from cardiac protein should not be used for measurements of the degradation of heme-containing proteins unless reutilization is controlled.

Original languageEnglish (US)
Pages (from-to)577-582
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume93
Issue number2
DOIs
StatePublished - Mar 28 1980

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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