Inhibition of protein synthesis with cycloheximide or puromycin caused a decrease in total cardiac proteolysis, as measured by the rate of release of radioactive phenylalanine from protein. At the same time, the rate of release of δ-aminolevulinic acid (δALA), which is incorporated specifically into heme-containing proteins, was increased significantly. These results suggest that, contrary to previously accepted beliefs, heme is extensively recycled in cardiac protein synthesis; therefore, the release of δALA from cardiac protein should not be used for measurements of the degradation of heme-containing proteins unless reutilization is controlled.
|Original language||English (US)|
|Number of pages||6|
|Journal||Biochemical and Biophysical Research Communications|
|State||Published - Mar 28 1980|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology