TY - JOUR
T1 - Increased release of δ-aminolevulinic acid from protein during inhibition of protein synthesis in heart
T2 - Evidence for the extensive reutilization of heme in cardiac protein metabolism
AU - Ord, John M.
AU - Wildenthal, Kern
PY - 1980/3/28
Y1 - 1980/3/28
N2 - Inhibition of protein synthesis with cycloheximide or puromycin caused a decrease in total cardiac proteolysis, as measured by the rate of release of radioactive phenylalanine from protein. At the same time, the rate of release of δ-aminolevulinic acid (δALA), which is incorporated specifically into heme-containing proteins, was increased significantly. These results suggest that, contrary to previously accepted beliefs, heme is extensively recycled in cardiac protein synthesis; therefore, the release of δALA from cardiac protein should not be used for measurements of the degradation of heme-containing proteins unless reutilization is controlled.
AB - Inhibition of protein synthesis with cycloheximide or puromycin caused a decrease in total cardiac proteolysis, as measured by the rate of release of radioactive phenylalanine from protein. At the same time, the rate of release of δ-aminolevulinic acid (δALA), which is incorporated specifically into heme-containing proteins, was increased significantly. These results suggest that, contrary to previously accepted beliefs, heme is extensively recycled in cardiac protein synthesis; therefore, the release of δALA from cardiac protein should not be used for measurements of the degradation of heme-containing proteins unless reutilization is controlled.
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U2 - 10.1016/0006-291X(80)91116-X
DO - 10.1016/0006-291X(80)91116-X
M3 - Article
C2 - 7387660
AN - SCOPUS:0018901878
SN - 0006-291X
VL - 93
SP - 577
EP - 582
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 2
ER -