Increased substrate affinity in the Escherichia coli L28R dihydrofolate reductase mutant causes trimethoprim resistance

Physics & Astronomy

• Escherichia 88%
• affinity 80%
• inhibitors 60%
• causes 44%
• enzymes 33%
• mutations 24%
• hydrogen bonds 21%
• drugs 21%
• products 20%
• public health 15%
• purines 15%
• combat 14%
• glutamates 14%
• titration 13%
• metabolism 12%
• maintenance 10%
• heat measurement 9%
• dissociation 8%
• entropy 7%
• synthesis 7%
• molecular dynamics 7%
• water 5%
• cells 5%
• simulation 3%

Chemical Compounds

• Trimethoprim 100%
• Dihydrofolates 92%
• Resistance 46%
• Antifolate 19%
• Mutation 11%
• Tetrahydrofolate 9%
• Kcat/Km 8%
• Hydrogen Bond 8%
• Isothermal Titration Calorimetry 7%
• Drug 7%
• Purine 6%
• Entropy 5%
• Pharmacological Metabolism 5%
• Kd 5%
• Molecular Dynamics 4%
• Dissociation 4%
• Simulation 4%
• Reduction 3%
• Time 2%