Increased synthesis and production of prostaglandin E2 by monocytes from drug addicts with AIDS

E. Fernandez-Cruz, E. Gelpi, N. Longo, B. Gonzalez, M. T. De la Morena, M. Garcia Montes, J. Rosello, I. Ramis, A. Suarez, A. Fernandez, J. M. Zabay

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Abstract

We analysed the production of prostaglandin E2 (PGE2) by monocytes In 11 drug addicts with AIDS and opportunistic infections and in 13 controls. In three patients and six controls we isolated the PGE2 fraction from culture supernatants of purified monocytes (> 95%) incubated in medium containing (3H) arachidonic acid, using silicic acid columns. In two of three patients PGE2-fraction values (21.9 and 21.6pmol/g protein) were significantly higher than controls (10 ± 4). In eight AIDS patients and seven controls, PGE2 levels were determined by high-performance liquid chromatography and radioimmunoassay (HPLC and RIA). In three out of eight patients PGE2 levels were markedly higher (736, 419 and 208pg/ml) than the mean + 2 s.d. values from controls (73 ± 51). We tested the effect of PGE2 on the production of interleukin-2 by normal phytohemagglutinin-stimulated peripheral blood lymphocytes (PBL). There was a significant suppression of interleukin-2 production by PGE2 released from AIDS monocytes. PGE2 may be one of the factors involved in the severe immune dysfunction associated with AIDS.

Original languageEnglish (US)
Pages (from-to)91-96
Number of pages6
JournalAIDS
Volume3
Issue number2
StatePublished - 1989

Keywords

  • AIDS
  • Drug addicts
  • HIV infection
  • Interleukin 2 production
  • Prostaglandin E<inf>2</inf> synthesis by monocytes
  • Prostaglandins

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology
  • Infectious Diseases

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    Fernandez-Cruz, E., Gelpi, E., Longo, N., Gonzalez, B., De la Morena, M. T., Garcia Montes, M., Rosello, J., Ramis, I., Suarez, A., Fernandez, A., & Zabay, J. M. (1989). Increased synthesis and production of prostaglandin E2 by monocytes from drug addicts with AIDS. AIDS, 3(2), 91-96.