Increased Toll-like receptor (TLR) mRNA expression in monocytes is a feature of metabolic syndrome in adolescents

O. T. Hardy, A. Kim, C. Ciccarelli, L. L. Hayman, J. Wiecha

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Background: Metabolic syndrome (MetSyn) is diagnosed frequently in some but not all overweight adolescents. Chronic inflammation, as seen in obesity, is strongly associated with MetSyn. Objectives: The aim of this pilot study was to assess the correlation between activation of the innate immune system and MetSyn, independent of body mass index (BMI), in a young population. Methods: We quantitatively measured both systemic pro-inflammatory cytokines and gene expression of Toll-like receptors (TLRs) and downstream cytokines in circulating monocytes obtained from nine adolescents with metabolic syndrome (Overwt-MetSyn) and eight BMI-matched controls (Overwt-Healthy). Results: The Overwt-MetSyn group demonstrated a significant elevation in expression of TLR2, TLR4, tumour necrosis factor-a (TNF a) and interleukin-6 (IL6) in peripheral monocytes, and increased circulating levels of TNF a and IL6 when compared with the Overwt-Healthy group. TLR2 (r = 0.78, P < 0.001), TLR4 (r = 0.57, P < 0.01) and TNF a (r = 0.61, P < 0.01) gene expression positively correlated with serum levels of TNF a. Conclusions: Our study suggests that activation of the innate immune pathway via TLRs may be partially responsible for the increased systemic inflammation seen in adolescents with MetSyn.

Original languageEnglish (US)
JournalPediatric obesity
Volume8
Issue number1
DOIs
StatePublished - Feb 2013

Fingerprint

Toll-Like Receptors
Monocytes
Messenger RNA
Tumor Necrosis Factor-alpha
Interleukin-6
Body Mass Index
Cytokines
Inflammation
Gene Expression
Immune System
Obesity
Serum
Population

Keywords

  • Adolescents
  • Innate immunity
  • Metabolic syndrome
  • Monocytes

ASJC Scopus subject areas

  • Health Policy
  • Pediatrics, Perinatology, and Child Health
  • Public Health, Environmental and Occupational Health
  • Nutrition and Dietetics

Cite this

Increased Toll-like receptor (TLR) mRNA expression in monocytes is a feature of metabolic syndrome in adolescents. / Hardy, O. T.; Kim, A.; Ciccarelli, C.; Hayman, L. L.; Wiecha, J.

In: Pediatric obesity, Vol. 8, No. 1, 02.2013.

Research output: Contribution to journalArticle

@article{eb0f130910ec4a7cbe8f6ad6baf1a534,
title = "Increased Toll-like receptor (TLR) mRNA expression in monocytes is a feature of metabolic syndrome in adolescents",
abstract = "Background: Metabolic syndrome (MetSyn) is diagnosed frequently in some but not all overweight adolescents. Chronic inflammation, as seen in obesity, is strongly associated with MetSyn. Objectives: The aim of this pilot study was to assess the correlation between activation of the innate immune system and MetSyn, independent of body mass index (BMI), in a young population. Methods: We quantitatively measured both systemic pro-inflammatory cytokines and gene expression of Toll-like receptors (TLRs) and downstream cytokines in circulating monocytes obtained from nine adolescents with metabolic syndrome (Overwt-MetSyn) and eight BMI-matched controls (Overwt-Healthy). Results: The Overwt-MetSyn group demonstrated a significant elevation in expression of TLR2, TLR4, tumour necrosis factor-a (TNF a) and interleukin-6 (IL6) in peripheral monocytes, and increased circulating levels of TNF a and IL6 when compared with the Overwt-Healthy group. TLR2 (r = 0.78, P < 0.001), TLR4 (r = 0.57, P < 0.01) and TNF a (r = 0.61, P < 0.01) gene expression positively correlated with serum levels of TNF a. Conclusions: Our study suggests that activation of the innate immune pathway via TLRs may be partially responsible for the increased systemic inflammation seen in adolescents with MetSyn.",
keywords = "Adolescents, Innate immunity, Metabolic syndrome, Monocytes",
author = "Hardy, {O. T.} and A. Kim and C. Ciccarelli and Hayman, {L. L.} and J. Wiecha",
year = "2013",
month = "2",
doi = "10.1111/j.I2047T-6310.201Y2.00098.x",
language = "English (US)",
volume = "8",
journal = "Pediatric obesity",
issn = "2047-6310",
publisher = "Wiley-Blackwell for the International Association for the Study of Obesity",
number = "1",

}

TY - JOUR

T1 - Increased Toll-like receptor (TLR) mRNA expression in monocytes is a feature of metabolic syndrome in adolescents

AU - Hardy, O. T.

AU - Kim, A.

AU - Ciccarelli, C.

AU - Hayman, L. L.

AU - Wiecha, J.

PY - 2013/2

Y1 - 2013/2

N2 - Background: Metabolic syndrome (MetSyn) is diagnosed frequently in some but not all overweight adolescents. Chronic inflammation, as seen in obesity, is strongly associated with MetSyn. Objectives: The aim of this pilot study was to assess the correlation between activation of the innate immune system and MetSyn, independent of body mass index (BMI), in a young population. Methods: We quantitatively measured both systemic pro-inflammatory cytokines and gene expression of Toll-like receptors (TLRs) and downstream cytokines in circulating monocytes obtained from nine adolescents with metabolic syndrome (Overwt-MetSyn) and eight BMI-matched controls (Overwt-Healthy). Results: The Overwt-MetSyn group demonstrated a significant elevation in expression of TLR2, TLR4, tumour necrosis factor-a (TNF a) and interleukin-6 (IL6) in peripheral monocytes, and increased circulating levels of TNF a and IL6 when compared with the Overwt-Healthy group. TLR2 (r = 0.78, P < 0.001), TLR4 (r = 0.57, P < 0.01) and TNF a (r = 0.61, P < 0.01) gene expression positively correlated with serum levels of TNF a. Conclusions: Our study suggests that activation of the innate immune pathway via TLRs may be partially responsible for the increased systemic inflammation seen in adolescents with MetSyn.

AB - Background: Metabolic syndrome (MetSyn) is diagnosed frequently in some but not all overweight adolescents. Chronic inflammation, as seen in obesity, is strongly associated with MetSyn. Objectives: The aim of this pilot study was to assess the correlation between activation of the innate immune system and MetSyn, independent of body mass index (BMI), in a young population. Methods: We quantitatively measured both systemic pro-inflammatory cytokines and gene expression of Toll-like receptors (TLRs) and downstream cytokines in circulating monocytes obtained from nine adolescents with metabolic syndrome (Overwt-MetSyn) and eight BMI-matched controls (Overwt-Healthy). Results: The Overwt-MetSyn group demonstrated a significant elevation in expression of TLR2, TLR4, tumour necrosis factor-a (TNF a) and interleukin-6 (IL6) in peripheral monocytes, and increased circulating levels of TNF a and IL6 when compared with the Overwt-Healthy group. TLR2 (r = 0.78, P < 0.001), TLR4 (r = 0.57, P < 0.01) and TNF a (r = 0.61, P < 0.01) gene expression positively correlated with serum levels of TNF a. Conclusions: Our study suggests that activation of the innate immune pathway via TLRs may be partially responsible for the increased systemic inflammation seen in adolescents with MetSyn.

KW - Adolescents

KW - Innate immunity

KW - Metabolic syndrome

KW - Monocytes

UR - http://www.scopus.com/inward/record.url?scp=84878310934&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84878310934&partnerID=8YFLogxK

U2 - 10.1111/j.I2047T-6310.201Y2.00098.x

DO - 10.1111/j.I2047T-6310.201Y2.00098.x

M3 - Article

C2 - 22991262

AN - SCOPUS:84878310934

VL - 8

JO - Pediatric obesity

JF - Pediatric obesity

SN - 2047-6310

IS - 1

ER -