Increased type I procollagen mRNA in airways and pulmonary vessels after vagal denervation in rats

Terry W. Carver, Sadeesh K. Srinathan, Christine R. Velloff, J. Julio Pérez Fontán

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

To test the hypothesis that increased airway strain resulting from lung denervation initiates a fibroproliferative response in the airways, we compared the transcriptional expressions of α1(I)-procollagen and tropoelastin in the lungs of rats subjected to unilateral vagal denervation, unilateral vagal denervation combined with ipsilateral phrenectomy, or thoracotomy without denervation (controls). We found increases in α1(I)-procollagen messenger ribonucleic acids (mRNAs) in the submucosa of the airways and the adventitia of airways and pulmonary vessels of the denervated lungs in 31% of the rats subjected to unilateral denervation (with and without phrenectomy), and in none of the controls. The increased transcripts were associated with collagen deposition in the peribronchial and perivascular tissue, and occasionally with cell proliferation leading to occlusion of the airway and vascular lumina. Unilateral phrenectomy did not decrease the frequency with which production of Type I procollagen was upregulated, suggesting that the upregulation was not entirely dependent on airway strain. Tropoelastin expression was not influenced by denervation. Our results indicate that the autonomic nervous system has a previously unsuspected trophic influence on collagen synthesis in the airways and pulmonary vessels. Abolition of this influence by denervation may lead to structural changes analogous to those observed in bronchiolitis obliterans after lung transplantation.

Original languageEnglish (US)
Pages (from-to)691-701
Number of pages11
JournalAmerican Journal of Respiratory Cell and Molecular Biology
Volume17
Issue number6
StatePublished - 1997

Fingerprint

Denervation
Collagen Type I
Rats
RNA
Lung
Tropoelastin
Procollagen
Collagen
Bronchiolitis Obliterans
Adventitia
Lung Transplantation
Autonomic Nervous System
Cell proliferation
Neurology
Thoracotomy
Blood Vessels
Up-Regulation
Cell Proliferation
Tissue

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Pulmonary and Respiratory Medicine

Cite this

Increased type I procollagen mRNA in airways and pulmonary vessels after vagal denervation in rats. / Carver, Terry W.; Srinathan, Sadeesh K.; Velloff, Christine R.; Pérez Fontán, J. Julio.

In: American Journal of Respiratory Cell and Molecular Biology, Vol. 17, No. 6, 1997, p. 691-701.

Research output: Contribution to journalArticle

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