Increases in intracellular calcium of arterial smooth muscle cells in transgenic mice overexpressing Na+/H+ exchanger

Jun Ichi Kiraku, Takao Sugiyama, Terunao Ashida, Naoyuki Takahashi, Jun Fujii, Makoto Kuro-o, Ryozo Nagai

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We produced transgenic mice overexpressing Na+/H+ exchanger as a model of salt-sensitive hypertension and reported that dietary salt loading elevates blood pressure in these transgenic mice. We speculate that this blood pressure elevation may be attributed to the elevation of intraarterial smooth muscle Ca2+ concentration through Na+/Ca2+ exchange. To test this hypothesis, we measured the isometric tension of aortic rings and intracellular free calcium ([Ca2+](i)) of cultured smooth muscle cells. In the transgenic mice, the aortic ring contraction induced by 5 mM caffeine (percentage of 60 mM K-induced contraction) was significantly greater than control mice (60.1 ± 5.5% vs. 44.8 ± 3.1%). The mean [Ca2+](i) in vascular smooth muscle cells (VSMCs) of transgenic mice (123.1 ± 19.7 nM) was higher than those in VSMCs of control mice (66.6 ± 7.2 nM). These observations suggest that dietary salt loading increases the concentration of calcium in arterial smooth muscle cells in this transgenic mice. These findings are helpful in tracing the causes of salt-sensitive hypertension.

Original languageEnglish (US)
Pages (from-to)511-513
Number of pages3
JournalJournal of Cardiovascular Pharmacology
Volume35
Issue number3
DOIs
StatePublished - 2000

Fingerprint

Sodium-Hydrogen Antiporter
Transgenic Mice
Smooth Muscle Myocytes
Calcium
Salts
Vascular Smooth Muscle
Blood Pressure
Hypertension
Caffeine
Smooth Muscle
Arterial Pressure

Keywords

  • Calcium
  • Hypertension
  • Salt
  • Sodium-proton exchanger

ASJC Scopus subject areas

  • Pharmacology
  • Cardiology and Cardiovascular Medicine

Cite this

Increases in intracellular calcium of arterial smooth muscle cells in transgenic mice overexpressing Na+/H+ exchanger. / Kiraku, Jun Ichi; Sugiyama, Takao; Ashida, Terunao; Takahashi, Naoyuki; Fujii, Jun; Kuro-o, Makoto; Nagai, Ryozo.

In: Journal of Cardiovascular Pharmacology, Vol. 35, No. 3, 2000, p. 511-513.

Research output: Contribution to journalArticle

Kiraku, Jun Ichi ; Sugiyama, Takao ; Ashida, Terunao ; Takahashi, Naoyuki ; Fujii, Jun ; Kuro-o, Makoto ; Nagai, Ryozo. / Increases in intracellular calcium of arterial smooth muscle cells in transgenic mice overexpressing Na+/H+ exchanger. In: Journal of Cardiovascular Pharmacology. 2000 ; Vol. 35, No. 3. pp. 511-513.
@article{9af06dcb02914b7c829c83a788556f80,
title = "Increases in intracellular calcium of arterial smooth muscle cells in transgenic mice overexpressing Na+/H+ exchanger",
abstract = "We produced transgenic mice overexpressing Na+/H+ exchanger as a model of salt-sensitive hypertension and reported that dietary salt loading elevates blood pressure in these transgenic mice. We speculate that this blood pressure elevation may be attributed to the elevation of intraarterial smooth muscle Ca2+ concentration through Na+/Ca2+ exchange. To test this hypothesis, we measured the isometric tension of aortic rings and intracellular free calcium ([Ca2+](i)) of cultured smooth muscle cells. In the transgenic mice, the aortic ring contraction induced by 5 mM caffeine (percentage of 60 mM K-induced contraction) was significantly greater than control mice (60.1 ± 5.5{\%} vs. 44.8 ± 3.1{\%}). The mean [Ca2+](i) in vascular smooth muscle cells (VSMCs) of transgenic mice (123.1 ± 19.7 nM) was higher than those in VSMCs of control mice (66.6 ± 7.2 nM). These observations suggest that dietary salt loading increases the concentration of calcium in arterial smooth muscle cells in this transgenic mice. These findings are helpful in tracing the causes of salt-sensitive hypertension.",
keywords = "Calcium, Hypertension, Salt, Sodium-proton exchanger",
author = "Kiraku, {Jun Ichi} and Takao Sugiyama and Terunao Ashida and Naoyuki Takahashi and Jun Fujii and Makoto Kuro-o and Ryozo Nagai",
year = "2000",
doi = "10.1097/00005344-200003000-00024",
language = "English (US)",
volume = "35",
pages = "511--513",
journal = "Journal of Cardiovascular Pharmacology",
issn = "0160-2446",
publisher = "Lippincott Williams and Wilkins",
number = "3",

}

TY - JOUR

T1 - Increases in intracellular calcium of arterial smooth muscle cells in transgenic mice overexpressing Na+/H+ exchanger

AU - Kiraku, Jun Ichi

AU - Sugiyama, Takao

AU - Ashida, Terunao

AU - Takahashi, Naoyuki

AU - Fujii, Jun

AU - Kuro-o, Makoto

AU - Nagai, Ryozo

PY - 2000

Y1 - 2000

N2 - We produced transgenic mice overexpressing Na+/H+ exchanger as a model of salt-sensitive hypertension and reported that dietary salt loading elevates blood pressure in these transgenic mice. We speculate that this blood pressure elevation may be attributed to the elevation of intraarterial smooth muscle Ca2+ concentration through Na+/Ca2+ exchange. To test this hypothesis, we measured the isometric tension of aortic rings and intracellular free calcium ([Ca2+](i)) of cultured smooth muscle cells. In the transgenic mice, the aortic ring contraction induced by 5 mM caffeine (percentage of 60 mM K-induced contraction) was significantly greater than control mice (60.1 ± 5.5% vs. 44.8 ± 3.1%). The mean [Ca2+](i) in vascular smooth muscle cells (VSMCs) of transgenic mice (123.1 ± 19.7 nM) was higher than those in VSMCs of control mice (66.6 ± 7.2 nM). These observations suggest that dietary salt loading increases the concentration of calcium in arterial smooth muscle cells in this transgenic mice. These findings are helpful in tracing the causes of salt-sensitive hypertension.

AB - We produced transgenic mice overexpressing Na+/H+ exchanger as a model of salt-sensitive hypertension and reported that dietary salt loading elevates blood pressure in these transgenic mice. We speculate that this blood pressure elevation may be attributed to the elevation of intraarterial smooth muscle Ca2+ concentration through Na+/Ca2+ exchange. To test this hypothesis, we measured the isometric tension of aortic rings and intracellular free calcium ([Ca2+](i)) of cultured smooth muscle cells. In the transgenic mice, the aortic ring contraction induced by 5 mM caffeine (percentage of 60 mM K-induced contraction) was significantly greater than control mice (60.1 ± 5.5% vs. 44.8 ± 3.1%). The mean [Ca2+](i) in vascular smooth muscle cells (VSMCs) of transgenic mice (123.1 ± 19.7 nM) was higher than those in VSMCs of control mice (66.6 ± 7.2 nM). These observations suggest that dietary salt loading increases the concentration of calcium in arterial smooth muscle cells in this transgenic mice. These findings are helpful in tracing the causes of salt-sensitive hypertension.

KW - Calcium

KW - Hypertension

KW - Salt

KW - Sodium-proton exchanger

UR - http://www.scopus.com/inward/record.url?scp=0034003795&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034003795&partnerID=8YFLogxK

U2 - 10.1097/00005344-200003000-00024

DO - 10.1097/00005344-200003000-00024

M3 - Article

VL - 35

SP - 511

EP - 513

JO - Journal of Cardiovascular Pharmacology

JF - Journal of Cardiovascular Pharmacology

SN - 0160-2446

IS - 3

ER -