TY - JOUR
T1 - Independent pathways for secretion of cholesterol and apolipoprotein E by macrophages
AU - Basu, Sandip K.
AU - Goldstein, Joseph L.
AU - Brown, Michael S.
PY - 1983
Y1 - 1983
N2 - Cholesterol-loaded macrophages secrete cholesterol and apolipoprotein E. The current studies show that this secretion occurs by two independent pathways. In the absence of serum, the cells secrete apolipoprotein E, but not cholesterol. In the presence of monensin (an inhibitor of protein secretion), the cells secrete cholesterol, but little apolipoprotein E. After secretion, apolipoprotein E and cholesterol associate with high-density lipoprotein to form a particle that can deliver cholesterol to the liver by receptor-mediated endocytosis. We conclude that apolipoprotein E does not function to remove cholesterol from macrophages but rather to participate in "reverse cholesterol transport.".
AB - Cholesterol-loaded macrophages secrete cholesterol and apolipoprotein E. The current studies show that this secretion occurs by two independent pathways. In the absence of serum, the cells secrete apolipoprotein E, but not cholesterol. In the presence of monensin (an inhibitor of protein secretion), the cells secrete cholesterol, but little apolipoprotein E. After secretion, apolipoprotein E and cholesterol associate with high-density lipoprotein to form a particle that can deliver cholesterol to the liver by receptor-mediated endocytosis. We conclude that apolipoprotein E does not function to remove cholesterol from macrophages but rather to participate in "reverse cholesterol transport.".
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U2 - 10.1126/science.6823554
DO - 10.1126/science.6823554
M3 - Article
C2 - 6823554
AN - SCOPUS:0020665553
SN - 0036-8075
VL - 219
SP - 871
EP - 873
JO - Science
JF - Science
IS - 4586
ER -