Indocyanine green enhanced near infrared laser treatment of SCK tumors in a mouse model-pilot study

Gal Shafirstein, Wolfgang Bäumler, Ran Friedman, Leah Hennings Dvm, Jessica Webber, James Suen, Robert J. Griffin

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Background and Purpose. Determine the efficacy of indocyanine green (ICG) dye in enhancing near infrared (NIR) laser ablation of tumors in a mouse model. Methods. Mammary carcinoma cells of A/J mice were injected subcutaneously in the lower back of female A/J mice (n=6). Five to seven days post inoculation the tumors (7-9 mm) were treated with 755-nm laser using 70 J/cm2 radiant exposures and 3-ms pulse time. Epidermal cooling was accomplished by cryogen spray cooling. Two minutes prior to laser irradiation mice were injected, intravenously, with 4 mg/kg body weight of ICG solution. Results. Complete tumor ablation was observed in the tumor region and minor damage was seen in the healthy skin. No major skin damage was observed post treatment. Substantial damage (up to 100% coagulative necrosis) was observed in tissue collected from tumors that were treated with laser/ICG. Conclusions. Intravenous administration of 4 mg/kg ICG significantly enhanced thermal ablation of tumors during NIR laser irradiation while sparing healthy skin.

Original languageEnglish (US)
Title of host publicationEnergy-Based Treatment of Tissue and Assessment VI
DOIs
StatePublished - Apr 14 2011
Externally publishedYes
EventEnergy-Based Treatment of Tissue and Assessment VI - San Francisco, CA, United States
Duration: Jan 23 2011Jan 24 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume7901
ISSN (Print)1605-7422

Conference

ConferenceEnergy-Based Treatment of Tissue and Assessment VI
CountryUnited States
CitySan Francisco, CA
Period1/23/111/24/11

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging

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  • Cite this

    Shafirstein, G., Bäumler, W., Friedman, R., Dvm, L. H., Webber, J., Suen, J., & Griffin, R. J. (2011). Indocyanine green enhanced near infrared laser treatment of SCK tumors in a mouse model-pilot study. In Energy-Based Treatment of Tissue and Assessment VI [790112] (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 7901). https://doi.org/10.1117/12.876424