Inducible macropinocytosis of hyaluronan in B16-F10 melanoma cells

Henry J. Greyner, Tomasz Wiraszka, Li Shu Zhang, W. Matthew Petroll, Mark E. Mummert

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Hyaluronan (HA) is a glycosaminoglycan composed of N-acetylglucosamine and glucuronic acid subunits. Endocytosis is thought to play an essential role in the catabolism of HA due to the intracellular compartmentalization of the HA degrading hyaluronidase enzymes. Previous investigations have shown that keratinocytes, chondrocytes and breast tumor cell lines endocytose HA via the cell surface glycoprotein, CD44. However, other cell types endocytose HA using a CD44-independent mechanism that remains to be defined. The purpose of this study was to investigate HA endocytosis in B16-F10 melanoma cells. We found that B16-F10 melanoma cells expressed CD44 on their surfaces. Unexpectedly, CD44 did not play a role in the endocytosis of HA. Electron microscopy studies revealed that B16-F10 melanoma cells exhibited membrane ruffling, a characteristic feature of macropinocytosis, only after incubating the cells with the HA co-polymer. Moreover, B16-F10 melanoma cells endocytosed HA via macropinocytosis as assessed by drug inhibition studies and the co-localization of fluorescently labeled HA with fluorescent tracers under confocal microscopy. Based on these results, we conclude that induced macropinocytosis may provide a previously unrecognized avenue for HA endocytosis in some cell types.

Original languageEnglish (US)
Pages (from-to)503-510
Number of pages8
JournalMatrix Biology
Volume29
Issue number6
DOIs
StatePublished - Jul 2010

Fingerprint

Experimental Melanomas
Hyaluronic Acid
Endocytosis
Glucuronic Acid
Hyaluronoglucosaminidase
Acetylglucosamine
Membrane Glycoproteins
Chondrocytes
Glycosaminoglycans
Tumor Cell Line
Keratinocytes
Confocal Microscopy
Electron Microscopy
Polymers
Cell Membrane
Breast Neoplasms

Keywords

  • B16-F10 melanoma
  • CD44
  • Endocytosis
  • Hyaluronan
  • Macropinocytosis

ASJC Scopus subject areas

  • Molecular Biology

Cite this

Inducible macropinocytosis of hyaluronan in B16-F10 melanoma cells. / Greyner, Henry J.; Wiraszka, Tomasz; Zhang, Li Shu; Petroll, W. Matthew; Mummert, Mark E.

In: Matrix Biology, Vol. 29, No. 6, 07.2010, p. 503-510.

Research output: Contribution to journalArticle

Greyner, Henry J. ; Wiraszka, Tomasz ; Zhang, Li Shu ; Petroll, W. Matthew ; Mummert, Mark E. / Inducible macropinocytosis of hyaluronan in B16-F10 melanoma cells. In: Matrix Biology. 2010 ; Vol. 29, No. 6. pp. 503-510.
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