Induction by NGF of meiotic maturation of Xenopus oocytes expressing the trk proto-oncogene product

Gel R. Nebreda, Dionisio Martin-Zanca, David R. Kaplan, Luis F. Parada, Eugenio Santos

Research output: Contribution to journalArticlepeer-review

116 Scopus citations

Abstract

The effect of nerve growth factor (NGF) was assessed in Xenopus oocytes expressing the human trk proto-oncogene product, p140prototrk. Oocytes injected with trk messenger RNA expressed polypeptides recognized by antibodies to the trk gene product. Exposure of these oocytes to nanomolar amounts of NGF resulted in specific surface binding of 125I-labeled NGF, tyrosine phosphorylation of p140prototrk, and meiotic maturation, as determined by germinal vesicle breakdown and maturation promoting factor (p34cdc2) kinase activation. Thus the trk proto-oncogene product can act as a receptor for NGF in a functionally productive manner.

Original languageEnglish (US)
Pages (from-to)558-561
Number of pages4
JournalScience
Volume252
Issue number5005
DOIs
StatePublished - 1991

ASJC Scopus subject areas

  • General

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