Induction by NGF of meiotic maturation of Xenopus oocytes expressing the trk proto-oncogene product

Gel R. Nebreda, Dionisio Martin-Zanca, David R. Kaplan, Luis F. Parada, Eugenio Santos

Research output: Contribution to journalArticle

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Abstract

The effect of nerve growth factor (NGF) was assessed in Xenopus oocytes expressing the human trk proto-oncogene product, p140prototrk. Oocytes injected with trk messenger RNA expressed polypeptides recognized by antibodies to the trk gene product. Exposure of these oocytes to nanomolar amounts of NGF resulted in specific surface binding of 125I-labeled NGF, tyrosine phosphorylation of p140prototrk, and meiotic maturation, as determined by germinal vesicle breakdown and maturation promoting factor (p34cdc2) kinase activation. Thus the trk proto-oncogene product can act as a receptor for NGF in a functionally productive manner.

Original languageEnglish (US)
Pages (from-to)558-561
Number of pages4
JournalScience
Volume252
Issue number5005
StatePublished - Apr 26 1991

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Proto-Oncogenes
Oncogene Proteins
Nerve Growth Factor
Xenopus
Oocytes
Maturation-Promoting Factor
Nerve Growth Factor Receptor
Tyrosine
Phosphotransferases
Phosphorylation
Messenger RNA
Peptides
Antibodies
Genes

ASJC Scopus subject areas

  • General

Cite this

Nebreda, G. R., Martin-Zanca, D., Kaplan, D. R., Parada, L. F., & Santos, E. (1991). Induction by NGF of meiotic maturation of Xenopus oocytes expressing the trk proto-oncogene product. Science, 252(5005), 558-561.

Induction by NGF of meiotic maturation of Xenopus oocytes expressing the trk proto-oncogene product. / Nebreda, Gel R.; Martin-Zanca, Dionisio; Kaplan, David R.; Parada, Luis F.; Santos, Eugenio.

In: Science, Vol. 252, No. 5005, 26.04.1991, p. 558-561.

Research output: Contribution to journalArticle

Nebreda, GR, Martin-Zanca, D, Kaplan, DR, Parada, LF & Santos, E 1991, 'Induction by NGF of meiotic maturation of Xenopus oocytes expressing the trk proto-oncogene product', Science, vol. 252, no. 5005, pp. 558-561.
Nebreda GR, Martin-Zanca D, Kaplan DR, Parada LF, Santos E. Induction by NGF of meiotic maturation of Xenopus oocytes expressing the trk proto-oncogene product. Science. 1991 Apr 26;252(5005):558-561.
Nebreda, Gel R. ; Martin-Zanca, Dionisio ; Kaplan, David R. ; Parada, Luis F. ; Santos, Eugenio. / Induction by NGF of meiotic maturation of Xenopus oocytes expressing the trk proto-oncogene product. In: Science. 1991 ; Vol. 252, No. 5005. pp. 558-561.
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