TY - JOUR
T1 - Induction in the developing compound eye of Drosophila
T2 - Multiple mechanisms restrict R7 induction to a single retinal precursor cell
AU - Van Vactor, David L.
AU - Cagan, Ross L.
AU - Krämer, Helmut
AU - Zipursky, S. Lawrence
N1 - Funding Information:
We would like to thank Bill Biggs for assistance with the XA12 line, Joseph OTousa for the DfninaPr7,e line, Seymour Benzer for MAb 22Cl0, and B. E. Kimmel, M. E. Fortini, and G. M. Rubin for antibodies to rough protein and the Rh4JecZ construct. For enhancer trap insertions at the seven-up locus, we thank M. Mlodzik, Y. Hlromi, C. S. Goodman, and G. M. Rubin. We would also like to thank members of the Zipursky lab, and Drs. E. DeRobertis, S. Crews, and U. Banerjee for editorial suggestions. This research was supported by the Howard Hughes Medical Institute and the following grants: an National Institute of Mental HealthlNRSA Fellowship (D. V. V), a National Institutes of Health Postdoctoral Fellowship (Ft. C.), an EMBO Postdoctoral Felfow-ship (H. K.), an American Cancer Society Faculty Research Award (S. L. 2.). a McKnight Foundation Development Award (S. L. Z.), and a National Institutes of Health Grant.
PY - 1991/12/20
Y1 - 1991/12/20
N2 - The development of the Drosophila R7 photoreceptor cell is determined by a specific inductive interaction between the R8 photoreceptor cell and a single neighboring precursor cell. This process is mediated by bride of sevenless (boss), a cell-surface bound ligand, and the sevenless (sev) tyrosine kinase receptor. The boss ligand is expressed specifically on the surface of the R8 cell, whereas the sev receptor is expressed on 5 cells contacting the developing R8 cell and other cells not in contact with R8. By altering the spatial and temporal expression of boss, we demonstrate that sev-expressing cells that do not contact R8 can assume an R7 cell fate. By contrast, the sev-expressing precursor cells to the R1-R6 photoreceptor cells that do contact R8 are nonresponsive to the inductive cue. Using the rough and Nspl mutations, we demonstrate that an early commitment to an R1-R6 cell fate blocks the pathway of sev activation in these cells.
AB - The development of the Drosophila R7 photoreceptor cell is determined by a specific inductive interaction between the R8 photoreceptor cell and a single neighboring precursor cell. This process is mediated by bride of sevenless (boss), a cell-surface bound ligand, and the sevenless (sev) tyrosine kinase receptor. The boss ligand is expressed specifically on the surface of the R8 cell, whereas the sev receptor is expressed on 5 cells contacting the developing R8 cell and other cells not in contact with R8. By altering the spatial and temporal expression of boss, we demonstrate that sev-expressing cells that do not contact R8 can assume an R7 cell fate. By contrast, the sev-expressing precursor cells to the R1-R6 photoreceptor cells that do contact R8 are nonresponsive to the inductive cue. Using the rough and Nspl mutations, we demonstrate that an early commitment to an R1-R6 cell fate blocks the pathway of sev activation in these cells.
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U2 - 10.1016/0092-8674(91)90291-6
DO - 10.1016/0092-8674(91)90291-6
M3 - Article
C2 - 1760842
AN - SCOPUS:0026332452
SN - 0092-8674
VL - 67
SP - 1145
EP - 1155
JO - Cell
JF - Cell
IS - 6
ER -