Induction of B cell differentiation by T cell factors. I. Stimulation of IgM secretion by products of a T cell hybridoma and a T cell line

E. Pure, P. C. Isakson, K. Takatsu, T. Hamaoka, S. L. Swain, R. W. Dutton, G. Dennert, J. W. Uhr, E. S. Vitetta

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60 Scopus citations

Abstract

We have attempted to determine the requirements for slg and T cell factors in the polyclonal activation of murine B lymphocytes. Highly enriched normal (neonatal or adult) or neoplastic B cells were cultured with T cell supernatants in the absence or presence of Sepharose-coupled anti-μ or anti-δ and assayed for polyclonal IgM secretion. The T cell supernatants were derived from Con A pulsed normal spleen cells, the C.C3.11.75 T cell line stimulated with antigen, or the B151K12 hybridoma (not pulsed with Con A). Unlike the spleen cell supernatants, the C.C3.11.75 and B151K12 supernatants lack Interleukin-1 (IL-1) and Interleukin-2 (IL-2); all 3 supernatants contain T cell-replacing factor (TRF). We have confirmed previous data which demonstrated that a) anti-Ig induces proliferation of mature B cells, and b) terminal differentiation to IgM secretion requires the presence of both anti-Ig and supernatant from Con A-pulsed spleen cells (CAS). In addition, we have shown that immature B cells, which do not proliferate in response to anti-Ig, can be induced to secrete IgM by anti-Ig plus CAS. In contrast to supernatants from Con A-pulsed spleen cells, supernatants from the C.C3.11.75 and B151K12 cell lines alone induced polyclonal IgM secretion by all populations of B cells tested; this was not accompanied by detectable proliferation. Furthermore, the polyclonal responses induced by the supernatants from the line and the hybridoma were not enhanced by anti-Ig. These results suggest that there are at least 2 pathways for T cell-induced B cell differentiation in vitro and that these pathways may involve different subpopulations of B cells. The 1st pathway requires 2 signals: 1 is provided by soluble macrophage or T cell factor(s) and the other is provided by cross-linking of slg. The 2nd pathway involves differentiation of some B cells in response to T cell help alone. These pathways can be distinguished by the source of the T cell supernatants.

Original languageEnglish (US)
Pages (from-to)1953-1958
Number of pages6
JournalJournal of Immunology
Volume127
Issue number5
StatePublished - Dec 14 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Pure, E., Isakson, P. C., Takatsu, K., Hamaoka, T., Swain, S. L., Dutton, R. W., Dennert, G., Uhr, J. W., & Vitetta, E. S. (1981). Induction of B cell differentiation by T cell factors. I. Stimulation of IgM secretion by products of a T cell hybridoma and a T cell line. Journal of Immunology, 127(5), 1953-1958.