Induction of CD8 T cells by vaccination with recombinant adenovirus expressing human papillomavirus type 16 E5 gene reduces tumor growth

D. W. Liu, Y. P. Tsao, C. H. Hsieh, Jer-Tsong Hsieh, J. T. Kung, C. L. Chiang, S. J. Huang, S. L. Chen

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The potential of the E5 protein as a tumor vaccine candidate has not been explored yet. In this study, we evaluate the human papillomavirus type 16 (HPV-16) E5 protein delivered by an adenovirus vector as a tumor vaccine for cervical lesions. The results demonstrate that a single intramuscular injection of a recombinant adenovirus carrying the HPV-16 E5 gene into syngeneic animals can reduce the growth of tumors which contain E5 gene expression. Moreover, the E5 vaccine-induced tumor protection occurs through CD8 T cells but not through CD4 T cells in in vitro assays. In addition, our studies using knockout mice with distinct T-cell deficiencies confirm that cytotoxic T-lymphocyte-induced tumor protection is CD8 dependent but CD4 independent. Hence, HPV-16 E5 can be regarded as a tumor rejection antigen.

Original languageEnglish (US)
Pages (from-to)9083-9089
Number of pages7
JournalJournal of Virology
Volume74
Issue number19
DOIs
StatePublished - Jan 1 2000
Externally publishedYes

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Cancer Vaccines
Human papillomavirus 16
Papillomaviridae
Adenoviridae
Vaccination
T-lymphocytes
vaccination
T-Lymphocytes
neoplasms
Growth
Genes
Neoplasms
genes
Intramuscular Injections
Cytotoxic T-Lymphocytes
Neoplasm Antigens
vaccines
Knockout Mice
Gene Expression
cytotoxic T-lymphocytes

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Induction of CD8 T cells by vaccination with recombinant adenovirus expressing human papillomavirus type 16 E5 gene reduces tumor growth. / Liu, D. W.; Tsao, Y. P.; Hsieh, C. H.; Hsieh, Jer-Tsong; Kung, J. T.; Chiang, C. L.; Huang, S. J.; Chen, S. L.

In: Journal of Virology, Vol. 74, No. 19, 01.01.2000, p. 9083-9089.

Research output: Contribution to journalArticle

Liu, D. W. ; Tsao, Y. P. ; Hsieh, C. H. ; Hsieh, Jer-Tsong ; Kung, J. T. ; Chiang, C. L. ; Huang, S. J. ; Chen, S. L. / Induction of CD8 T cells by vaccination with recombinant adenovirus expressing human papillomavirus type 16 E5 gene reduces tumor growth. In: Journal of Virology. 2000 ; Vol. 74, No. 19. pp. 9083-9089.
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