TY - JOUR
T1 - Induction of cell spreading by substratum-adsorbed ligands directed against the cell surface
AU - Grinnell, Frederick
AU - Hays, Donald G.
N1 - Funding Information:
We are indebtedt o Dr Richard Anderson for his helpful criticism of this manuscript. This research was supported by grants from the NIH. CA14609 and GM21698.
PY - 1978/10/15
Y1 - 1978/10/15
N2 - Studies were carried out to compare the spreading of baby hamster kidney (BHK) cells, which occurs by an interaction between the cells and a specific serum glycoprotein (ASF) adsorbed onto the substratum surface, with the spreading of BHK cells that occurs by an interaction between the cells and substrata coated with ligands directed at various cell surface determinants. The ligands tested were polycationic ferritin, concanavalin A (ConA) and antibody directed against BHK plasma membranes. Cell spreading onto ASF and ligand-coated substrata were similar even though different cell surface components were apparently involved. The similarities were: 1. 1. The shape of the spread cells. 2. 2. The inhibition of cell spreading by conditions that interfere with metabolic activity, block free sulfhydryl groups, or interfere with microtubules and microfilaments. 3. 3. The similar reorganization of certain cell surface antigenic determinants during cell spreading onto any of the substrata. The results indicate that cell spreading is a general cellular response to specific cell-substratum interactions but does not depend upon binding between a unique cell surface receptor and the substratum.
AB - Studies were carried out to compare the spreading of baby hamster kidney (BHK) cells, which occurs by an interaction between the cells and a specific serum glycoprotein (ASF) adsorbed onto the substratum surface, with the spreading of BHK cells that occurs by an interaction between the cells and substrata coated with ligands directed at various cell surface determinants. The ligands tested were polycationic ferritin, concanavalin A (ConA) and antibody directed against BHK plasma membranes. Cell spreading onto ASF and ligand-coated substrata were similar even though different cell surface components were apparently involved. The similarities were: 1. 1. The shape of the spread cells. 2. 2. The inhibition of cell spreading by conditions that interfere with metabolic activity, block free sulfhydryl groups, or interfere with microtubules and microfilaments. 3. 3. The similar reorganization of certain cell surface antigenic determinants during cell spreading onto any of the substrata. The results indicate that cell spreading is a general cellular response to specific cell-substratum interactions but does not depend upon binding between a unique cell surface receptor and the substratum.
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U2 - 10.1016/0014-4827(78)90449-4
DO - 10.1016/0014-4827(78)90449-4
M3 - Article
C2 - 710527
AN - SCOPUS:0018173470
SN - 0014-4827
VL - 116
SP - 275
EP - 284
JO - Experimental Cell Research
JF - Experimental Cell Research
IS - 2
ER -