Induction of cell spreading by substratum-adsorbed ligands directed against the cell surface

Frederick Grinnell, Donald G. Hays

Research output: Contribution to journalArticle

56 Scopus citations

Abstract

Studies were carried out to compare the spreading of baby hamster kidney (BHK) cells, which occurs by an interaction between the cells and a specific serum glycoprotein (ASF) adsorbed onto the substratum surface, with the spreading of BHK cells that occurs by an interaction between the cells and substrata coated with ligands directed at various cell surface determinants. The ligands tested were polycationic ferritin, concanavalin A (ConA) and antibody directed against BHK plasma membranes. Cell spreading onto ASF and ligand-coated substrata were similar even though different cell surface components were apparently involved. The similarities were: 1. 1. The shape of the spread cells. 2. 2. The inhibition of cell spreading by conditions that interfere with metabolic activity, block free sulfhydryl groups, or interfere with microtubules and microfilaments. 3. 3. The similar reorganization of certain cell surface antigenic determinants during cell spreading onto any of the substrata. The results indicate that cell spreading is a general cellular response to specific cell-substratum interactions but does not depend upon binding between a unique cell surface receptor and the substratum.

Original languageEnglish (US)
Pages (from-to)275-284
Number of pages10
JournalExperimental Cell Research
Volume116
Issue number2
DOIs
StatePublished - Oct 15 1978

ASJC Scopus subject areas

  • Cell Biology

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