Induction of colonic epithelial cell apoptosis by p47-dependent oxidants

Ying Gu, Rhonda F. Souza, Ru Feng Wu, You Cheng Xu, Lance S. Terada

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Exogenous oxidants appear capable of initiating both proliferative and death signals, but the role of endogenous oxidants in either tumorigenesis or tumor suppression is unclear. We found that expression of the NAD(P)H oxidase adapter p47phox was suppressed in human colon carcinoma specimens relative to adjacent normal colon. Overexpression of p47phox increased apoptosis in colon cancer cell lines independent of p53 and mismatch-repair competency. p47phox was found to interact with the c-Abl adapter Abl interactor-1 (ABI-1), and p47phox coprecipitated with both ABI-1 and c-Abl. Ectopic expression of p47phox in colon cancer cells increased oxidant production with phosphorylation and activation of nuclear c-Abl and consequent apoptosis. Colonic epithelial p47phox may be specifically targeted to a c-Abl-containing complex that serves a physiologic tumor suppressing function.

Original languageEnglish (US)
Pages (from-to)195-200
Number of pages6
JournalFEBS Letters
Volume540
Issue number1-3
DOIs
StatePublished - Apr 10 2003

Keywords

  • Abl interactor-1
  • Colon cancer
  • NADPH oxidase
  • Oxidants
  • Superoxide
  • c-Abl

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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